摘要
为一氧化氮(NO)和乙烯(ET)在打破杜梨种子休眠中的作用及信号关系,用硝普钠(SNP)、乙烯利(ETH)、NO清除剂PTIO、ET作用抑制剂1-甲基环丙烯(1-MCP)等处理休眠的杜梨种子后,在20℃下用培养皿滤纸法培养,观察种子萌发率。结果表明,NO和ET均能有效地打破杜梨种子休眠,其中10mmol·L-1SNP和2mmol·L-1 ETH 24h浸泡处理使杜梨种子14d时的发芽率分别达到55.7%和57.8%,与对照相比差异显著(p<0.05),PTIO具逆转SNP作用的特性,1-MCP具逆转ETH作用的特性;经SNP和ETH处理后,可显著提高杜梨种子内源ET的释放速率,分别使杜梨种子第4天ET的释放速率达到0.14μL·kg-1·h-1和0.15μL·kg-1·h-1,显著高于对照(0.04μL·kg-1·h-1),1-MCP可以逆转SNP打破休眠的作用,但是PTIO不能逆转ETH的作用。说明NO和ET都能打破杜梨种子休眠,NO处在ET信号通路的上游,通过促进内源ET的形成来打破杜梨种子休眠。
The effects and signal relationships of nitric oxide（NO）and ethylene on breaking the seed dormancy of Pyrus betulifolia were examined. The dormancy seeds were treated with sodium nitroprusside（SNP）, ethephon（ETH）, NO scavenger 2-phenyl-4, 4, 5, 5-tetramethylimidazo-line-1-oxyl-3-oxide（PTIO）, and ethylene inhibitor 1-methylcyclo-propene（1-MCP）individually or with the combination. The treated seeds were then trained by dish-filter method at 20℃, and the seed germination rate was observed. The results indicated that both NO and ET could effectively break the seed dormancy, by which the germination rates of 24-hour pretreated seeds treated by 10 mmol·L-1SNP or 2 mmol·L-1 ETH were 55.7% and 57.8%, respectively, significantly different with the control（p〈0.05）. PTIO reversed the role of SNP, and 1-MCP reversed the role of ETH. By the treatment of SNP and ETH, endogenous ethylene production of the seeds significantly increased, the release rates of ethylene reached 0.14 μL·kg-1·h-1 and 0.15 μL·kg-1·h-1, respectively at the 4th day of the treatment by SNP and ETH, significantly higher than that of the control（0.04 μL·kg-1·h-1）. 1-MCP could reverse the above effects of SNP, however, no evidence were found for PTIO to reverse the effects of ETH, indicating that the either NO or ethylene could break the seed dormancy, and NO was at the up stream of ethylene signaling pathway, involving the stimulation of ethylene production in breaking P. betulifolia seed dormancy.
出处
《西北林学院学报》
CSCD
北大核心
2013年第6期51-55,共5页
Journal of Northwest Forestry University
基金
陕西省农业科技创新项目(2012NKC01-22)
关键词
杜梨种子
休眠
一氧化氮
乙烯
Pyrus betulifolia seed
dormancy
NO
ethylene
