摘要
目的 探讨乳腺癌转移抑制基因1（BRMS1）在胶质瘤细胞黏附和迁移中的作用和机制.方法 脂质体介导真核表达质粒pEGFP-BRMS1和对照质粒pEGFP-N2转入人胶质瘤U251和U87细胞中,Western blot技术检测BRMS1蛋白的表达.黏附实验和迁移实验检测细胞黏附和迁移能力.Western blot检测磷酸化黏着斑激酶（FAK） （p-FAK）和磷酸化酪氨酸激酶（Src）（p-Src）蛋白的表达.结果 与对照组比较,转染pEGFP-BRMS1质粒24 h后U251和U87细胞中BRMS1蛋白表达显著增高;细胞黏附能力分别下降60％和65％;细胞穿越Transwell小室能力分别下降64％和68％,差异均有统计学意义（P＜0.01）;转染BRMS1后两种细胞p-FAK和p-Src表达量明显减少.结论 BRMS1通过减少p-FAK/p-Src表达抑制胶质瘤细胞黏附和迁移能力.
Objective To investigate the effect and mechanism of breast cancer metastasis suppressor 1 （BRMS1） on the invasion of human glioma cells.Methods The pEGFP-BRMS1 plasmids and pEGFP-N2 control plasmids were transfected into the U251 and U87 glioma cells using Lipofectamine 2000.The expression of BRMS1 mRNA and the expression levels of phosphorylated tyrosine kinase （Src） and focal adhesion kinase （FAK） proteins after BRMS1 restoration in both glioma cell lines were assessed by Western blotting.The roles of BRMS1 in the adhesion and migration of glioma cells were studied by using cell attachment assay and cell migration assay respectively.Results Compared to the control plasmids,pEGFP-BRMS1 plasmids could increase the expression of BRMS1 in both glioma cell lines significantly.Cell attachment assay revealed that BRMS1 inhibited the adhesion ability of U251 and U87 cells by 60％ and 63％,respectively （P ＜ 0.01）.Cell migration assay showed that the migration ability of U251BRMS1 cells and U87-BRMS1 cells was decreased by 62％ and 68％ respectively as compared with the control cells （P ＜ 0.01）.The phosphorylated Src and FAK expression levels were significantly suppressed after BRMS1 transfection into U251 and U87 cells as compared with the control cells.Conclusion BRMS1 may suppress the adhesion and migration ability of glioma cells by reducing the expression of phosphorylated FAK and Src.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第12期2606-2608,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81201636）
江苏省自然科学基金资助项目（BK2012146）
关键词
乳腺癌转移抑制基因1
胶质瘤
黏附
迁移
Breast cancer metastasis suppressor 1
Gioma
Adhesion
Migration
