摘要
目的探讨Toll样受体4(toll-like receptor,TLR4)蛋白的表达在颞叶癫痫发病机制中的作用。方法通过腹腔注射氯化锂-毛果芸香碱建立大鼠颞叶癫痫模型。将大鼠分为盐水对照组、无急性发作组、急性发作组、无自发性发作组、慢性自发性发作组,采用免疫组化法检测各组大鼠海马TLR4蛋白的表达水平。同时采用免疫组化法检测颞叶癫痫患者及对照组患者脑组织中TLR4蛋白的表达水平。对各组大鼠行脑电图(electro-encephalogram,EEG)监测。结果盐水对照组、无急性发作组、急性发作组、无慢性自发性发作组、慢性自发性发作组大鼠海马TLR4蛋白表达水平分别为0.07858±0.01098、0.08438±0.01422、0.13810±0.01264、0.08672±0.00701、0.19650±0.01386。慢性自发性发作组大鼠脑海马TLR4蛋白表达水平明显高于其他各组(P<0.05);TLR4蛋白在急性发作期有短暂的表达增高,但低于慢性自发性发作组(P<0.05);盐水对照组、无急性发作组、无慢性自发性发作组的TLR4蛋白表达水平比较无统计学差异(P>0.05)。同时,颞叶癫痫患者脑组织中TLR4蛋白表达水平(0.79100±0.01750)亦高于对照组(0.15700±0.02588)(P<0.05)。结论 TLR4蛋白的过度表达参与了颞叶癫痫的发病机制,TLR4蛋白在癫痫急性期发作后的表达失调可能诱发了癫痫的反复发作。
Objective To investigate the function of toll-like receptor 4 （TLR4） protein in the pathogenesis of temporal lobe epilepsy.Methods Chloride-pilocarpine was used to establish epileptic models by intraperitoneal injection.Rats were divided into five groups：the saline treated control group,the non-acute seizure group,the acute seizure group,the non-spontaneous recurrent seizure group and the spontaneous recurrent seizure group.The expression level of TLR4 protein in the hippocampus of rats was tested by immunohistochemistry.At the same time the expression of TLR4 protein in human slices was tested by immunohistochemistry.Electroencephalogram （EEG） was used as auxiliary measure.Results The mean TLR4 expression levels of the saline treated control group,non-acute seizure group,acute seizure group,non-spontaneous recurrent seizure group,spontaneous recurrent seizure group were 0.07858 ± 0.01098,0.08438±0.01422,0.13810 ± 0.01264,0.08672 ± 0.00701,0.19650 ± 0.01386.The expression of TLR4 protein in hippocampus of spontaneous recurrent seizure rats was significantly higher than those of other groups （P＜ 0.05）; TLR4 protein expression transitorily increased in the acute seizure group,but lower than that of the spontaneous recurrent seizure group （P＜0.05）.On the other hand,the expression level of TLR4 protein had no significant difference in the other three groups （P＞0.05）.Moreover,the expression of TLR4 protein was higher in the epilepsy human tissues （0.79100 ± 0.01750） compared with the nonepileptic human brain tissues （0.15700± 0.02588） （P＜ 0.05）.Conclusions The overexpression of TLR4 protein may be involved in the mechanism of temporal lobe epilepsy,the over expression of TLR4 protein after the onset of epileptic may induce repeated seizures.
出处
《中国神经免疫学和神经病学杂志》
CAS
北大核心
2013年第3期192-196,共5页
Chinese Journal of Neuroimmunology and Neurology
关键词
癫痫
颞叶
TOLL样受体4
氯化锂
毛果芸香碱
海马
epilepsy, temporal lobe
toll-like receptor 4
lithium chloride
pilocarpine
hippocampus
