摘要
目的调查本地区幼儿园儿童唾液中EB病毒携带情况,为预防和控制EB病毒感染提供依据。方法随机抽取本地区城区和郊区2所幼儿园儿童共385名,采集唾液样本,采用实时荧光定量PCR方法检测唾液中EBV DNA及载量。并检测其中183例儿童血清EBV抗体谱,结果进行感染分期,探讨儿童EBV感染分期与唾液EB病毒携带的关系。结果本地区385名幼儿园儿童唾液中EBV DNA阳性率为39.0%;男童唾液EBV DNA阳性率高于女童（P=0.014）;唾液EBV DNA载量平均值4.35＋1.04 lgCopies/ml,男女儿童唾液EBV DNA载量差异无统计学意义（P=0.818）。不同年龄儿童唾液EBV DNA阳性率差异有统计学意义（P〈0.0001）,从低年龄组到高年龄组呈上升趋势;不同年龄儿童组唾液EBV DNA载量差异有统计学意义（P〈0.0001）,以5岁最高,3岁最低。城区、郊区儿童唾液EBV DNA阳性率差异无统计学意义（P=0.950）,但城区儿童唾液EBV DNA载量高于郊区儿童（P〈0.0001）。183名3岁~6岁年龄儿童EB病毒感染率为63.9%,唾液中EBV DNA阳性率38.8%。既往感染例115例,唾液中EBV DNA阳性率为59.1%。结论监控传染源,避免传播途径的发生,延迟儿童初次感染年龄是EB病毒感染防治的重点。
Objective To investigate the carrying situation of the EB virus in the saliva of kindergarten children in this region,and provide basis for prevention and control of EB virus infection.Methods A total of 385 children were selected from two kindergartens in urban area and rural area to collect the saliva samples.The EBV DNA and EBV DNA load in these saliva samples were detected by real-time fluorescent quantitative PCR.Serum EBV antibody repertoire was detected in 183 children,and the results were conducted infection staging to discuss the relationship between EBV infection staging and EBV carrying situation in saliva.Results In 385 kindergarten children of this area,the positive rate of EBV DNA was 39.0% in saliva,higher in boys than that in girls（P= 0.014）; the average EBV DNA load was 4.35＋1.04 Ig Copies/ml,and there was no statistical difference boys and girls（P=0.818）.EBV DNA positive rates showed uptrend from lower age group to higher age group with statistical difference（P0.0001）.EBV DNA load had statistical difference among children with different ages（P0.0001）,highest in 5-year-old children and lowest in 3-year-old children.In 183 children between 3 and 6 years old,the EBV infection rate was 63.9%,and EBV DNA positive rate was 38.8%.In 115 previous infection cases,the HBV DNA positive rate was 59.1% in saliva.Conclusion It is key to prevent and control EBV infection through monitoring Infection source,avoiding the route of transmission and delaying the first-infection age of children.
出处
《中国卫生检验杂志》
北大核心
2013年第13期2792-2794,2797,共4页
Chinese Journal of Health Laboratory Technology
