摘要
以蓝羊草为试材,以持续浇水处理为对照,采用2因素完全随机设计,利用盆栽方法研究了干旱胁迫对蓝羊草相对含水量、渗透调节物质及抗氧化酶活性的影响。结果表明:叶片相对含水量随着干旱胁迫程度的加深而下降,2周后达到20%左右,叶片的保水性较好,植株的耐旱性较强;在自然失水的条件下丙二醛(MDA)含量的积累逐渐增多,而且后期升高较为明显,植株对干旱的反映较为敏感;脯氨酸(Pro)含量随着叶片失水也随之升高,含水量与Pro含量呈显著负相关(相关系数为-0.865,P=0.026<0.05),其含量的积累对于蓝羊草是伤害反应,与抗旱性无关;可溶性蛋白质含量在干旱胁迫下呈现出快速下降后缓慢升高的趋势,超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性与对照相比增加显著(P<0.05),过氧化物酶(POD)活性与对照差异极显著,在抵御干旱方面共同发挥作用。
With Leymus chinensis as experimental material, control was sustainable watering, this test adopted the two factors completely random design. The effect of drought stress on relative water content,osmotic adjustment substance and antioxidant enzymes activity were studied by pot experiment. The results showed that relative water content was decreased with aggravation of drought stress,reached about 20% after 2 weeks. Water retention of leaves was better,and with good drought tolerance. In the condition of natural dehydration the accumulation of MDA was increased gradually, particularly at later stages of drought, and plant was sensitive to drought. Pro content increased with the water stress time. A significant correlation existed between relative water content and Pro content（R=- 0. 865, P= 0. 026〈0. 05）. Accumulation of Pro content was a wound response for plant, and it was independent of drought resistance. Under drought stress soluble protein content increased at first but later decreased. SOD and CAT activity in control were lower than other treatments （P〈0. 05） ,and both of them played a common role in preventing drought. POD activity in control was extremely lower than other treatments （P〈0. 01）.
出处
《北方园艺》
CAS
北大核心
2013年第23期71-75,共5页
Northern Horticulture
基金
2013年北京市属高等学校人才强教深化计划-创新人才(教学名师)建设资助项目(PXM2013_157203_000005)
关键词
蓝羊草
干旱胁迫
完全随机
抗氧化酶
Leymus chinensis
drought stress
completely random
antioxidant enzymes
