摘要
以不同种（品种）泡桐叶片为材料，利用第二代高通量测序技术和生物信息学方法构建泡桐小RNA（sRNA）文库，并对其microRNA（miRNA）靶基因功能进行分析。结果表明：在得到的87066707条高质量reads中，能与泡桐转录组完全匹配的有41399208条，占高质量序列的47．55％。sRNA长度主要分布在20—25nt之间，其中，长度为24nt的数量最多，其次为21nt的sRNA。鉴定出的44个保守miRNA分属于14个不同miRNA家族，并且不同miRNA家族的miRNA成员数量也存在差异。对鉴定出的27个新miRNA进行靶基因预测及功能分析，结果为阐明miRNAs在泡桐生长发育过程中的作用奠定基础。
A sRNA library of the mixed leaves of different Paulownia species and varieties was constructed with the second generation high-throughput sequencing technology and bioinformatics and the functions of microRNA （miRNAs） were analyzed in this paper. The results showed that the 41 399 208 out of the obtained 87 066 707 high-quality reads were perfectly aligned to transcription of Paulownia plant in the small RNAs library, accounting for 47.55%. The length of sRNAs ranged mainly from 20 to 25 ut, and the sRNA of 24 nt was the most abundant, followed by 21 nt reads. The 44 conserved miRNAs, belonging to 14 miRNA families, were identified, and there was difference in miRNA numbers of various miRNAs families. Moreover, functions of the target genes of 27 novel miRNAs were analyzed. These results provided a basis for clarifying miRNA functions during growth and development of Paulownia plants.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2013年第11期77-82,共6页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(30271082
30571496
U1204309)
河南省杰出人才计划项目(122101110700)
郑州市科技创新团队资金项目(121PCXTD515)
