摘要
通过形态观察、巢式PCR和SSR分析,研究了不同浓度硫酸二甲酯(DMS)处理对毛泡桐丛枝病幼苗植原体及泡桐DNA的SSR扩增位点变化的影响。结果表明:质量浓度为15 mg/L的硫酸二甲酯处理5 h或质量浓度大于25 mg/L的硫酸二甲酯处理3 h以上仍成活的幼苗其形态上可转变为健康幼苗,但15 mg/L硫酸二甲酯处理5 h时形态健康的幼苗仍有丛枝病植原体的存在,而其余浓度处理后存活的幼苗内则检测不到植原体;质量浓度大于150 mg/L的硫酸二甲酯处理3 h以上的幼苗全部死亡。此外,毛泡桐丛枝病幼苗、健康幼苗和DMS处理后形态健康幼苗DNA的SSR扩增位点相同。
The effect of different concentration of dimethyl sulphate（DMS） on the morphological and phytoplasma changes of Paulownia tomentosa seedlings infected by witches＇ broom and DNA base sequences changes at SSR level were investigated by morphological observation,net-PCR and SSR analysis.The results indicated that the seedlings infected by witches＇ broom which were treated with optimal concentration DMS might be cured,however there were still exist phytoplasma after treated with 15 mg/L DMS for 5 h.The seedlings were not alive when DMS concentration was larger than 150 mg/L.Moreover,the DNA loci at SSR level of the healthy,infected and the infected-treated seedlings were the same.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第3期5-8,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金项目(30571496)
高等学校博士学科点专项科研基金项目(20050466003)
中国博士后基金项目(20070410874)
