摘要
目的 探讨深低温冻存角膜复温后内皮细胞活性变化的规律。方法 冻存牛角膜复温后 ,分别行器官培养(A组 )及optisol保存 (B组 ) ,检测内皮细胞密度、存活率 (ESR)及形态。结果 A组培养 6h密度下降了 71 2 % ,2 4hESR明显下降 (2 7 2 % ) ;B组保存 72h密度下降了 73 6% ,ESR明显下降 (3 7 2 % )。结论 深低温冻存可造成内皮细胞的潜伏性损伤 ,判定其活性应在复温后组织培养 6h后进行。
ObjectiveTo investigate the regulation of endothelium viability of cryopreserved corneas by the type of postthawing procedures.MethodsFrozen corneas were divided into two groups after thawing,and then were cultured in dMEM medium containing 15% FCS at 37℃ in Group I,while were stored in Optisol medium at 4℃ in Group Ⅱ.Endothelium survival rate(ESR) was evaluated by the staining of trypan blue and alizarin red.Endothelium density and morphology were assessed by specular microscope.ResultsIn Group Ⅰ,endothelium density lost 71 2% at 6h after postthawing and ESR reduced apparently(27 2%)at 24 h after postthawing in dMEM medium organ culture.In Group Ⅱ,endothelium density lost 73 6% and ESR reduced apparently(37 2%) at 72 h postthawing after storage in Optisol medium.ConclusionsLatent cell damage induced by freeing and thawing could occur on cryopreserved corneas.Reliable information about the viability of corneal endothelial cells should be derived from morphologic evaluation of the tissue 6 h after postthawing organ culture.Optisol medium storage might alleviate latent cell damage when frozen corneas were stored in Optisol medium after thawing.
出处
《眼科研究》
CSCD
2000年第6期515-517,共3页
Chinese Ophthalmic Research
