摘要
目的探讨癌基因。ｃ－ｅｒｂＢ－２产物、抑癌基因ｐ５３突变型蛋白的过度表达以及增殖细胞核抗原标记指数（ＰＣＮＡ－ＬＩ）与乳腺浸润性癌临床病理指标的联系及其预后价值。方法随机收集７９例临床随访资料齐备的病人，用免疫组化ＡＢＣ法检测其ｃ－ｅｒｂＢ－２、ｐ５３和ＰＣＮＡ的表达情况，用Ｃｏｘ回归分析其预后意义。结果ｃ－ｅｒｂＢ－２和ｐ５３过度表达的阳性率分别为３１．７％和４８．１％。ＰＣＡＮ－ＬＩ中位数为１７．６％。ｃ－ｅｒｂＢ－２与ｐ５３过度表达之间以及其与ＰＣＮＡ－ＬＩ、肿瘤大小、组织学分级、腋淋巴结状态等指标间均无明确的相互关联。ＰＣＮＡ－ＬＩ与组织学分级呈正相关，但高ＰＣＮＡ－ＬＩ与不良预后无明显关联。Ｃｏｘ回归分析显示，腋淋巴结、ｃ－ｅｒｂＢ－２和ｐ５３过度表达等３项指标反映不良预后，危险度分别是３．３０６、１．４１９６、１．４９８４。结论ｃ－ｅｒｂＢ－２和ｐ５３过度表达是两项危险度仅次于腋淋巴结状态的反映乳腺浸润性癌不良预后的指标，ＰＣＮＡ－ＬＩ无预后价值。
Objective To investigate the prognostic importance of p53, c-erbB-2 and PCNA in patients with invasive breast carcinoma. Methods Tissue Samples from 79 cases of breast cancer with full follow-up data were collected. Expression o f PCNA,overexpression of c-erbB-2 and mutant p53 protein was evaluated by immunohistochemistry in formalin-fixed paraffin-embedded tissues. The three new indicators and other more traditional clinicopathological parameters were analyzed by Cox regression model. Results of the 79 breast cancer specimens, 31. 7 % were c-erbB-2 positive and 48. 1 % were p53 positive. No significant association was found among the overexpression of c-erbB-2 and mutant p53,age at diagnosis, menopause, tumor size, histological grade, axillary lymph node involvement and PCNA labeling index(PCNA-LI). The median of PCNA-LI was 17. 6%. PCNA-LI was positively correlated to histological grade. In both monovariate Kaplan-Meier plot and multivariate Cox regression analysis,involvement of axillary lymph node,overexpression of c-erbB-2 and mutant p53, but not PCNA-LI, showed a positive correlation with shorter overall survival time. The hazard ratios of axillary lymph node involvement,overexpression of c-erbB-2 and p53 were 3. 306, 1. 4196, 1. 4984 respectively. Conclusion overexpression of c-erbB-2 and mutant p53 could be two useful indicators for poor prognosis in invasive breast carcinoma. PCNA-LI didn't showed significant prognositic value.
出处
《实用肿瘤杂志》
CAS
北大核心
2000年第6期377-381,共5页
Journal of Practical Oncology
