摘要
目的应用中空纤维液相微萃取(HF-LPME)技术建立血液样品中氯丙嗪的气相色谱分析方法。方法对影响氯丙嗪HF-LPME的实验条件进行优化,优化的萃取条件为:采用聚偏氟乙烯中空纤维膜,以正辛醇为萃取溶剂,于搅拌速率为2500 rpm条件下在8 ml碱化后的样品溶液中萃取25 min,取1μl萃取液气相色谱进行分析。结果该方法的线性范围为0.01 mg/L^10 mg/L,最低检出限0.01 mg/L,富集倍数为12.6倍,相对回收率93%~102%,RSD9.2%~13.3%。结论建立的HF-LPME方法操作简单、高效、所需有机溶剂少,可避免残留和交叉污染,适用于血液中氯丙嗪的检测。
Objective A method for determination of chlorpromazine in blood samples using hollow fiber -liquid phase micro - extraction （ HF - LPME） coupled with gas chromatography （GC） was developed. Methods The extraction conditions of chlorpromazine in blood were optimized. The polyvinylidene fluoride hollow fiber membrane （ PVDF - HF） and n - caprylic alcohol were employed. The sample solutions were alkalified and extracted at a stirring rate of 2500 rpm for 25 min. Results The linear range for chlorpromazine in blood was 0.01 mg/L - 10 mg/L, the detection limit （LOD） was 0.01 mg/L, the enrichment factors was 12.6, the recovery was 93% - 102%, RSD were 9.2% - 13.3%. Conclusion The HF - LPME method is simple, efficient, requiring less solvent and can avoid the residue and cross contamination, it is suitable for extraction and detection of chlorpromazine in blood samples.
出处
《中国卫生检验杂志》
北大核心
2013年第15期3015-3017,共3页
Chinese Journal of Health Laboratory Technology
基金
山西省科技攻关计划项目(20110321082)
山西医科大学青年基金项目(2010057389)
山西医科大学大学生创新项目(201371)
关键词
法医毒物分析
中空纤维液相微萃取
气相色谱法
氯丙嗪
血液
Forensic toxicological analysis
Hollow fiber - liquid phase micro - extraction
GC
Chlorpromazine
Blood
