摘要
目的探讨在大鼠海洛因戒断、脱毒治疗和复吸期间,胰岛淀粉样多肽(IAPP)和葡萄糖转运蛋白2(GLUT2)在胰岛β细胞内表达的变化。方法正常雄性SD大鼠32只,随机分为实验组(n=24)和正常对照组(n=8),实验组又分为海洛因依赖戒断组、美沙酮脱毒治疗组和海洛因复吸组。各组大鼠取胰腺组织,采用免疫组织化学SABC法及图像分析方法进行研究。结果海洛因戒断时IAPP和GLUT2阳性细胞免疫染色较正常对照组变深,平均灰度值均低于正常组(P<0.05),IAPP面数密度增大(P<0.05);美沙酮脱毒期间,IAPP和GLUT2免疫阳性细胞染色变浅,平均灰度值较戒断时升高,与正常组差异无统计学意义,IAPP面数密度于正常组比较无明显差异(P>0.05);海洛因复吸期间,IAPP和GLUT2阳性细胞免疫染色又加深,平均灰度值降低(P<0.05),IAPP面数密度再次增大(P<0.05)。结论在海洛因戒断和复吸期间,表达于大鼠胰岛β细胞的IAPP和GLUT2合成增加,美沙酮脱毒期间合成减少,两者变化规律一致,提示在海洛因戒断,脱毒和复吸过程中,两者的合成同步,可能参与调节海洛因戒断,美沙酮脱毒治疗及复吸大鼠机体能量代谢过程,与调节大鼠摄食、体重及保护胰岛细胞功能有关。
Objective To investigate the expression changes of islet amyloid polypeptiole （IAPP） and glucose transporter 2（GLUT2） in islet β cells during heroin withdrawal induced by naloxone, methadone detoxification treatment, and heroin relapse. Methods Male SD rats （ n = 32） were randomly divided into a normal control group （ NCG, n = 8） and an experiment group （ EG, n = 24）. The EG was further divided into heroin withdrawal group （HWG） , methadone detoxication group （ MDG ） , and heroin relapse group （ HRG ）. Pancreas was drawn from each group. Immunohistochemistry and image analysis were used to detect the expression of IAPP and GLUT2. Results As compared with the normal control, the immunostainning of IAPP and GLUT2 was stronger in β cells of islets from heroin withdrawal and relapse rats than that in controls ; the numerical density on area of IAPP also increased ; the mean gray values decreased in these two groups. No difference was found in the immunostainning, the mean gray values and the numerical density on area during detoxification treatment compared to controls. Conclusion During heroin withdrawal and relapse, the synthesis of IAPP and GLUT2 increased, while they became decreased in methadone detoxification. The changes of IAPP and GLUT2 were consistent and had same rhythm in three stages. IAPP and GLUT2 took part in energy metabolic process, which may be related to food intake and body weight, or protective function to β cells.
出处
《解剖学报》
CAS
CSCD
北大核心
2013年第6期795-799,共5页
Acta Anatomica Sinica
基金
贵州省社会发展科技攻关计划基金资助[黔科合S字(2007)1048]
