摘要
目的观察清幻灵对谷氨酸功能低下小鼠模型自主活动及学习记忆的影响。方法将昆明种小鼠60只随机分为5组，其中一组作为空白组，其余4组连续腹腔注射2周MK-801造模。2周后随机编为模型组、清幻灵小剂量组、清幻灵中剂量组、清幻灵大剂量组，给予相应药物灌胃1个月，同时采用自主活动仪、避暗仪、跳台仪分别观察5组的偏好行为、学习记忆的变化。结果模型组与空白组相比，自主活动明显增多，差异具有统计学意义（34．2±4．9次VS10．2±4．0次，t=5．54，P〈0．05）；避暗法实验结果显示：在第2周末，清幻灵各剂量组与空白组相比，潜伏期有所延长，但差异无统计学意义（P〉0．05）。在第6周末，清幻灵小、中、大剂量组的潜伏期与空白组相比，有不同程度的延长[（269．9±23．7）S，（270．0±17．5）S，（264．6±45．4）SVS（241．2±7．8）s]。其中，在第6周末清幻灵中剂量组、大剂量组潜伏期比第2周末分别延长了15．1s、19．3S。结论清幻灵对小鼠高活动性有正常调节作用，同时对小鼠学习记忆能力具有增强和改善作用，并且呈剂量依赖性。
Objective To investigate the effect of Qinghuanling （QHL） on autonomic activities and learning and memory of the schizophrenia mice model. Methods 60 Kunming mice were randomly di- vided into five groups, one group as blank group injected physiological saline, and the rest groups were injected MK- 801 continuously 14 days. Then the model groups were randomly numbered., model group, QHL low dose group, QHL middle dose group, and QHL high dose group, given corresponding drugs for each group one month by gavage. At the same time, five groups＇ autonomous activities behav- ior and preference were observed by using the independent activity instrument, step--through instrument and platform. Results Compared with the blank group, activity of the rest model groups was increased （34.2±4.9 vs 10.2±4.0, t = 5.54,P 〈 0.05）. At the end of the second week, QHL each dose group compared with the blank group, the latency with different degree of extension, no statistical difference （P 〉 0.05）. At the end of the sixth week, the QHL middle dose group and high dose group compared to the blank group, the latency with statistical difference [ （269.9 ±23.7） seconds, （270. 0 ± 17.5） sec- onds,（264.6±45.4） seconds vs （241.2±7.8） seeonds]. Among them, at the end of the sixth week, QHL middle close group, high dose group latency than second weekend were extended by 15.1 seconds, 19.3 seconds. Conclusions The QHL middle dose group and the QHL high dose group can protect the normal autonomic activities, can also strength and improve the ability of learning and memory dose de- pendently in schizophrenia mice.
出处
《神经疾病与精神卫生》
2013年第5期436-439,共4页
Journal of Neuroscience and Mental Health
基金
国家自然科学基金项目（81202938）
陕西省科学技术研究发展计划项目（2011KJXX65,2012K16-05-01）
关键词
自主活动
谷氨酸功能低下小鼠模型
清幻灵
学习记忆
Schizophrenia mice model
Qinghuanling（QHL）
Autonomic activity
Learningand memory
