摘要
目的:探讨茶黄素对大鼠脑缺血再灌注损伤后神经细胞凋亡的影响及机制。方法:制作大鼠大脑中动脉栓塞模型,实验大鼠随机分为假手术组、缺血再灌注组、茶黄素治疗组。再灌注前舌下静脉给药,大鼠缺血2 h,于再灌注24 h后处死动物,采用免疫组织化学法和RT-PCR法检测缺血区脑组织Bax和Bcl-2表达。结果:大鼠脑缺血再灌注损伤后,茶黄素治疗组与模型组相比,Bax表达显著下调,Bcl-2的表达显著上调,差异有统计学意义(P<0.05)。结论:茶黄素对大鼠脑缺血再灌注损伤具有保护作用,其作用机制可能与抑制Bax、促进Bcl-2表达有关。
bral ischemia-re Objective：To investigate the effect of theaflavin on nerve cell apoptosis and its mechanism after cere- perfusion injury in a rat. Methods： Model of middle cerebral were randomly divided into 3 groups：sham-operated group, ischemia-reperfus artery occlusion was made. Male adult rats ion group, theaflavin-treated group. The 2 h-ischemia-and 24 h- ischemia reperfusion rats were killed, which were anesthetized and subjected to reperfusion sublin- gual intravenous administration. Immunohistochemistry and RT-PCR were used to detect the expression of Bax and Bcl- 2. Results：The expression of Bax in the brain tissues of theaflavin-treated group was lower than that of the ischemia- reperfusion group; the expression of Bcl-2 in the brain tissues of theaflavin-treated group was higher than that of the is- chemic group. There was statistically significant difference （ P 〈 0.05 ）. Conclusion： Theaflavin has protective effects on cerebral ischemic-reperfusion injury through inhibiting Bax expression and promoting Bcl-2 expression
出处
《山西职工医学院学报》
CAS
2013年第5期1-3,10,共4页
Journal of Shanxi Medical College for Continuing Education
