摘要
为研究地方土猪源副猪嗜血杆菌（Haemophilusparasuis，Hps）的分子遗传进化特性，应用PCR方法扩增河南地方分离株XY0501的16SrRNA基因，并进行遗传进化分析。结果显示，地方土猪源Hps的16SrRNA基因全长为822bp，与国内外参考菌株的核苷酸序列同源性为97．1％～99．3％；与血清5型参考菌株的核苷酸序列同源性高达99．3％；基于16SrRNA基因序列绘制的系统进化树显示，地方土猪源Hps与血清5型Hps参考株属于同一分支。研究结果证明，Hps可感染地方土猪并导致发病，感染来源有待进一步查明；16SrRNA基因在Hps的不同血清型中高度保守，且无品种差异。
To study the molecular genetic evolution characteristics of Haemophilus parasuis （Hps）, PCR was used to de- termine the 16S rRNA gene of the strain XY0501 isolated from local pigs in Henan province, and genetic evolution analysis was conducted. The results showed that the amplication of 16S rRNA of the isolate XY0501 was 822 bp, the nucleotide sequence similarity among the reference strains was 97. 1M to 99.3%, and 99.3M with the strain serotype 5. The phylogenetie tree based on 16S rRNA revealed that the Hps isolate XY0501 from local pigs belonged to the same branch with reference strain se- rotype 5. The results identified that the Hps infection could cause severe clinical symptoms in pigs, but infection source need to he further investigation,in addition, 16S rRNA of Hps of different serotypes was conserved and no species difference.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第11期157-160,共4页
China Animal Husbandry & Veterinary Medicine
基金
河南省高校科技创新人才支持计划(2011HASTIT009)
