摘要
目的 :对细菌性腹膜炎家兔血浆中几种急性期反应蛋白进行动态观察及与体温和内毒素的关系。 方法 :将 2ml细菌混悬液 (2 .4× 10 8个 /ml)、2ml自体全血和 6ml生理盐水混匀 ,对家免腹腔注射 ,分别于造模前及造模后 1、2、4、8、2 4、32、48、72h观察不同时相家兔体温和外周血中内毒素及 7个急性期反应蛋白的变化。 结果 :体温于造模后 2h明显上升 ,8h达高峰 ,最高为 41 3℃ ,之后逐渐下降 ,72h接近正常水平。模型死亡率为 2 7 2 7%。血浆内毒素在造模后 72h内出现两个高峰 ,分别在 2h和 32h。血浆α2 巨球蛋白和转铁蛋白造模后即开始下降 ,72h内出现两次低谷。纤维连接蛋白在原有水平上下波动。α 酸性糖蛋白、C 反应蛋白、铜蓝蛋白和触珠蛋白的变化趋势基本相似 ,72h内出现两个升高的峰。 结论 :触珠蛋白可以作为临床诊断感染、观察病程和判断预后的最敏感的生化指标。C 反应蛋白是急性期反应蛋白中变化最早的一种 。
To observe the relationship between the se rum levels of acute phase response proteins of rabbits with bacterial peritoniti s and their body temperature Methods:Rabbits were injected intraperitoneally with a mixture of 2 ml bacterial supernatant(2.4×10 8/ml),2 ml blood and 6 ml NS.A cute phase response proteins and lipopolyssaccharides were examined before and 1 ,2,4,8,24,48 and 72 hours after injection.Results:Alte rations of C reactive protein appeared most early and lapotoglobin was most sen sitive to infection among the several kinds of acute phase response proteins.Conclusion:C reactive protein and lapotoglobin are he lpful to forecast infectious complications and to diagnose infection.
出处
《中国中西医结合外科杂志》
CAS
2000年第6期392-395,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
