摘要
目的 建立一个稳定、简易、经济且在病理学,细菌学都与临床相接近的细菌性腹膜炎模型。方法 以10 %BaSO4 肉汤作佐剂,用1×10 8、3×10 8、1×10 9、2×10 9、4×10 9cfu/ml 5个浓度的大肠杆菌家兔腹腔注射,观察家兔一般情况、体温、外周血白细胞总数和中性粒细胞变化,记录死亡时间,存活家兔第10天处死,取大网膜病理检查。结果 5组家兔的白细胞总数和中性粒细胞百分比与给菌前比较有明显变化。3×10 8cfu/ml组的死亡率适中(4 5 % ) ,而且各个时间段均有死亡,体温升高持续时间最长,大网膜病理检查示有不同程度炎症细胞浸润,部分有脓肿形成。结论 以10 %BaSO4 肉汤作佐剂,用3×10 8cfu/ml的大肠杆菌腹腔注射家兔后,可以得到一个感染程度适中,病理改变和临床相似,可比性强,重复性好的细菌性腹膜炎模型。
Objective To establish a stable, simple, economical animal model of bacterial peritonitis, which is similar to clinic in pathology and bacteriology. Methods Mixtures containing 10% BaSO4 and 1×10 8, 3×10 8, 1×10 9, 2×10 9 or 4×10 9cfu/ml Escherichia coli were injected into the peritoneal cavity of rabbits, respectively. General conditions, body temperature, leukocyte count and the percentage of neutrophil of the rabbits were closely observed. The time when rabbits died was recorded, and the autopsy was performed immediately. The greater omentum was obtained for pathological examination. All survived rabbits were killed on 10 th day. Results The leukocyte count and the percentage of neutrophil of all rabbits changed obviously after the rabbits were injected with bacteria. The mortality of the group with 3×10 8 cfu/ml Escherichia coli was moderate (45%). Moreover, the rabbits in this group died in various periods after injection and had a longest fever time. The pathological examination of greater omentum revealed inflammatory cells infiltration and even abscesses formed in some. Conclusions A stable, simple, moderate infected animal model of bacterial peritonitis can be established in rabbits by the injection of 3×10 8 cfu/ml Escherichia coli with 10% BaSO4, which is similar to clinic in pathology and bacteriology.
出处
《浙江预防医学》
2005年第5期1-3,11,共4页
Zhejiang Journal of Preventive Medicine
基金
浙江省科技厅重大项目 (0 1110 3717)
