摘要
为了解辽宁省2007～2012年流行性风疹病毒基因特征，本研究用Vero／Slam细胞从辽宁省2007～2012年风疹暴发和散发患者的咽拭子标本中分离到145株风疹病毒（Rubella，RV），应用逆转录一聚合酶链反应（RT～PCR）扩增RV分离株E1基因的945个核苷酸片段，对扩增产物进行序列测定和分析。将辽宁省145株RV与世界卫生组织（WHO）RV13个基因型的32株参考株基于739个核苷酸片段的基因定型序列构建基因亲缘性关系树。结果提示，辽宁省2007-2012年145株RV分离株均属于1E基因型，核苷酸和氨基酸同源性为97．2％～100．0％和97．6％～100．0％。与2株wHO1E基因型参考株（Rvi／Dezhou．CHN／02，RVi／MYS／01）序列相比，核苷酸和氨基酸同源性分别为96．6％～99．2％和98．2％～100．0％．除RVi／Shenyang．Liaoning．CHN／13．n／13株病毒在E1基因的第246位发生了突变（Val246-Ala246）；RVi／Shenyang．Liaoning．CHN／13．11／4和RVi／Liaoyang．Liaoning．CHN／26．11／2株病毒在E1基因的第262发生相同突变（Asp262-Asn262）外，其他毒株在N-型糖基化位、血凝抑制位点和中和位点等重要抗原位点均未发生变化，抗原性稳定。辽宁省所有1E基因型风疹病毒在E1蛋白的第338位氨基酸发生相同突变（Leu338-Phe338）。本研究证实1E基因型为辽宁省RV的优势基因型，近6年来辽宁省风疹疫情是由1E基因型RV的多个传播链引起。
To analyze the genetic characterization of epidemic rubella virus strains isolated in Liaoning from 2007~2012, a total of 145 rubella virus strains were isolated using Vero/Slam cell line from the patients~ throat swabs during rubella outbreaks and sporadics cases in Liaoning Province from 2007 to 2012. Fragments of 945 nucleotides containing 1E gene from 145 rubella virus isolates were amplified by RT-PCR, the PCR products were sequenced and analyzed. Based on the 739 nucleotides of 1E gene, the phylogenetic trees were constructed with 39, WHO rubella reference strains of 13 genotypes downloaded from GenBank and 145 rubella virus strains. The results showed that the 145 rubella virus strains in 2007 ~2012 belonged to genotype 1E, nucleotide acids and amino acids similarities were 97.2% -100.0% and 97.6%-100. 0%, respectively. Compared to the 1E reference strains （Rvi/ Dezhou. CHN/02, RVi/ MYS/01）, the nucleotide acids and amino acids similarities were 96.6%-99.2% and 98. 2%-100.0%, respectively except for one amino acid change （Va1246-Ala246） of RVi/Shenyang. Liaoning. CHN/13.11/ 13, and Asp262-Asn262 of RVi/Shenyang. Liaoning. CHN/13.11/4 and RVi/Liaoyang. Liaoning. CHN/ 26.11/2. there had no change found in the important antigenic epitope sites, the hemagglutination inhibi- tion and neutralization epitopes of the other rubella viruses. All the 145 strains isolated had the same ami- no acid change （Leu338--Phe338） in E1 protein. These findings suggested that genotype 1E of rubella vi- rus was the predominant genotype in Liaoning province, the rubella prevailed in recent six years was main- ly caused by rubella viruses genotype 1E with multi-transmission routes.
出处
《病毒学报》
CAS
CSCD
北大核心
2013年第6期589-595,共7页
Chinese Journal of Virology
基金
国家科技重大专项"传染病监测技术平台"项目(项目编号:2012ZX10004209)
关键词
风疹病毒
基因特征
基因型
Rubella virus
Genetic characterization
Genotype
