摘要
目的观察葛根散对酒精性脂肪肝大鼠肝功能损伤的影响。方法将100只Wistar雄性大鼠随机分为6组,正常对照组、模型对照组、联苯双酯滴丸组及葛根散高、中、低剂量组。正常对照组正常饲养,其余5组建立酒精性脂肪肝大鼠模型。葛根散高、中、低剂量组分别以24.84、12.42、6.21 g/kg灌胃给药,联苯双酯滴丸组以3.6 mg/kg灌胃给药,正常对照组与模型对照组以0.9%氯化钠注射液10 mL/kg灌胃给药。均每日1次,连续灌胃30 d。比较大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)及总胆红素(TBiL)水平变化,并观察各组大鼠肝组织的病理变化。结果与正常对照组比较,模型对照组大鼠血清ALT、AST及TBiL水平均显著升高(P<0.05);与模型对照组比较,葛根散高、中、低剂量组及联苯双酯滴丸组血清ALT、AST及TBiL水平均有明显降低(P<0.05),且葛根散高剂量组降低水平与联苯双酯滴丸组相当(P>0.05),并优于葛根散中、低剂量组(P<0.05)。结论葛根散可显著降低酒精性脂肪肝模型大鼠血清ALT、AST及TBiL水平,其药物疗效作用强度具有一定的剂量依赖关系,对酒精性脂肪肝模型大鼠肝损伤有保护作用。
Objective To observe the effect of Gegen powder on alcoholic fatty liver dysfunction rats .Meth-ods 100 Wistar rats were randomly divided into six groups ,control group,model group,bifendate pills group,high-,middle-and low-dose Gegen powder group .Rats in control group were fed in normal feeding .Rats in the other five groups were established alcoholic fatty liver model .24.84 g/kg,12.42 g/kg and 6.21 g/kg Gegen powder and 3.6 mg/kg bifendate pills were given in different treatment groups .Rats in control group and model group received 10 ml/kg 0.9%sodium chloride injection for continuous lavage 30 d.The changes of ALT,AST and TBiL in serum of rats were observed .And the pathological changes of liver tissue were observed .Results The levels of ALT ,AST and TBiL in model group were increased as compared with those in control group （P0.05）.Conclusion Gegen powder can obviously decrease the levels of ALT ,AST and TbiL in alcoholic fatty liver dysfunction rats ,intensity of its efficacy is a dose -dependent manner ,and has protection on alcoholic fatty liver dysfunction rat model .
出处
《河北中医》
2013年第11期1703-1704,1708,共3页
Hebei Journal of Traditional Chinese Medicine
关键词
脂肪肝
酒精性
疾病模型
动物
大鼠
葛根
Alcoholic fatty liver
Disease models
Animal
Rat
Pueraria
