摘要
[目的]观察白桦脂醇对酒精性肝损伤的治疗作用。[方法]将大鼠随机分为6组,正常组、低剂量组、中剂量组、高剂量组、阳性对照组和模型组。采用白酒灌胃的方法建立大鼠酒精性肝病模型,给予白桦脂醇保护。检测血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP),高密度脂蛋白(HDL)、低密度脂蛋白(LDL)以及肝匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、还原性谷胱甘肽酶(GSH)、谷胱甘肽-S-转移酶(GST)、丙二醛(MDA)水平,同时做肝脏的病理切片观察。[结果]酒精性肝损伤的大鼠血清ALT、AST、ALP活性和LDL含量明显升高。肝匀浆中SOD、GSH-PX、GST活力明显降低;MDA含量明显升高;GSH含量明显降低。白桦脂醇能明显降低模型组大鼠血清中ALT、AST、ALP活性(与模型组比较:P<0.05);可明显提高酒精性肝损伤大鼠肝组织中SOD、GSH-PX、GST活性(P<0.01)和GSH含量(P<0.05);同时显著性降低肝组织中MDA含量(P<0.05)。阳性对照组(益肝灵组)大鼠血清中ALT、AST、ALP活性明显降低和CHO含量明显降低(与模型组比较:P<0.05);可明显提高酒精性肝损伤大鼠肝组织中SOD、GSH-PX、GST活性(P<0.01)和GSH含量(P<0.05);同时显著性降低肝组织中MDA含量(P<0.05)。白桦脂醇还能明显改善由酒精引起的肝组织脂肪样变和坏死。[结论]白桦脂醇能抑制酒精诱导脂质过氧化反应对肝组织的损伤,对酒精性肝损伤有较好的治疗作用。
[Objective]To study the therapeutic effects of betulin on alcoholic liver disease. [Methods]Rats were randomly divided into 6 groups ：normal group, low-dose group, middle-dose group, high-dose group, positive control group and model group. The alcoholic liver disease model rats were made by feeding alcohol. ,the rats in postive control group were administered with Sli, the rats in low-dose group, middle-dose group,high-dose group were administered with betulin. The level of ALT, AST, ALP, HDL, LDL in serum and SOD, GSH-PX, GSH, GST and MDA in liver tissue were measured. HE staining was used to observe the pathological changes of liver. [Results]Compared with rats in model group,betulin significantly increased the SOD level, the GSH-PX level and the GST level in liver tissue and the ALT level, the AST level and the ALP level in serum; and obviously decredsed the TG content and the MDAcontent. The hepatic injuries of rats in betulin group were recovered obviously. [Conclusion]Betulin has obviously therapeutic effects on alcoholic liver injury in rats, the mechanisms may contribute to reducing lipid peroxide.
出处
《预防医学论坛》
2010年第9期825-827,共3页
Preventive Medicine Tribune
