摘要
目的 探讨乌司他丁对腹主动脉阻断联合腹腔注射脂多糖内毒素（LPS）构建严重脓毒症大鼠肾脏NOD1受体表达和血IL-18水平的影响.方法 48只Wistar大鼠随机分为假手术组（SHAM组）、腹主动脉阻断联合腹腔注射LPS组（AAC＋ LPS组）、腹主动脉阻断联合腹腔注射LPS乌司他丁干预组（AAC＋ LPS＋U组）、腹主动脉阻断腹腔LPS生理盐水对照组（AAC＋ LPS＋ NS组）.大鼠于术后或干预后8h处死,收集血和大鼠肾脏,检测血中Cr、BUN水平,ELISA方法检测血中IL-18浓度,免疫组化方法检测肾脏NOD1蛋白的表达,RT-PCR方法检测肾脏NOD1 mRNA的表达.结果 NOD1受体mRNA和蛋白的表达,血中IL-18和Cr、BUN水平在AAC ＋LPS组和AAC＋LPS ＋NS组最强,AAC＋ LPS＋U组较前两组下降,差异有统计学意义（P＜0.05）.结论 乌司他丁可能通过抑制肾脏NOD1受体的表达,抑制血中IL-18的分泌从而在脓毒症时发挥抗炎和肾脏保护作用.
Objective To establish a rat model of sepsis by using abdominal aorta clamping （AAC） and intraperitoneal injection of lipopolysaccharide （LPS） and explore the effect of Ulinastatin on renal NOD1 receptor and serum interlukin-18 in the acute kidney injury.Methods Forty-eight Wistar rats were divided into four groups randomly：SHAM group,AAC ＋ LPS group,AAC ＋ LPS ＋ Ulinastatin group,AAC ＋ LPS ＋ NS group.Rats were sacrificed at the 8th hour after operation,serum and kidney tissues were collected for detecting the creatinine（Cr） and blood urea nitrogen（BUN） level.ELISA method was used to test interlukin-18（IL-18）level in serum.The expression of NOD1 in kidney was observed by immunohistochemical stainning and RT-PCR was used to detect the NOD1 mRNA level in kidney.Results The serum Cr,BUN and IL-18 levels and NOD1 mRNA/protein expression in kidney were significantly increased （P ＜0.05）in the AAC ＋ LPS group and AAC ＋ LPS ＋ NS group than those in SHAM group,however in the AAC ＋ LPS ＋ Ulinastatin group,those index were decreased compared with AAC ＋ LPS group and AAC ＋LPS ＋ NS group.Conclusion Ulinastatin could play a protective role in sepsis-induced acute kidney injury through inhibiting the NOD1 receptor expression and IL-18 secretion.
出处
《临床外科杂志》
2013年第9期691-693,共3页
Journal of Clinical Surgery
基金
广东省药学会天普基金资助项目（01201103）
