摘要
目的:观察硫化氢(hydrogen sulfide,H2S)对幽门螺杆菌(H.pylori)感染的GES-1细胞CSE,NF-κB及IL-8 mRNA表达及其形态学的影响,探讨其对H.pylori所致胃黏膜细胞炎症的作用及机制。方法:将GES-1细胞培养24 h后分为对照组(不加H.pylori及NaHS)、H.pylori组、NaHS组(又分为4个亚组,分别加入50,100,200或400μmol/L NaHS)和H.pylori+NaHS组(又分为4个亚组,分别加入H.pylori与50,100,200或400μmol/L NaHS),每组分别培养3,6,及12 h,用RT-PCR法检测各组GES-1细胞CSE,NF-κB及IL-8 mRNA表达,并分析其相关性。结果:H.pylori组CSE,NF-κB及IL-8 mRNA表达均较对照组增加(P<0.05),200μmol/L NaHS组和400μmol/L NaHS组CSE表达较对照组降低(P<0.05);而NaHS各组NF-κB和IL-8 mRNA表达与对照组比较差异无统计学意义(P>0.05);NaHS各组、H.pylori+200μmol/L NaHS组及H.pylori+400μmol/L NaHS组CSE,NF-κB及IL-8 mRNA表达均较H.pylori组降低(P<0.05);H.pylori组、H.pylori+200μmol/L NaHS组及H.pylori+400μmol/L NaHS组CSE,NF-κB及IL-8 mRNA表达之间均呈正相关(P<0.05)。结论:H.pylori诱导GES-1细胞NF-κB和IL-8 mRNA表达,并上调CSE mRNA表达;200和400μmol/L NaHS能抑制H.pylori感染诱导的GES-1细胞NF-κB和IL-8 mRNA表达,改善H.pylori感染所致的细胞形态学变化,对细胞起保护作用。
Objective： To investigate the effect of hydrogen sulfide （HzS） on the expression of CSE, NF-~cB, and IL-8 mRNA in GES- 1 cells with Helicobacter pylori （H. pylori） infection and to explore itsmechanism on gastric mucosa inflammation caused by H. pylori. Methods： GES-1 cells were cultured for 24 h and divided into a control group （neither H. pylori nor NariS）, an H. pylori group, a NariS group （which was further divided into 4 groups at 50, 100, 200, or 400 [anol/L NariS）, and H. pylori ＋ NariS group （which was further divided into 4 groups at 50, 100, 200, or 400 [arnol/L NariS）. Each group was then cultured for 3, 6, or 12 h. The expression of CSE, NF-kB, and IL-8 mRNA was measured by RT-PCR, and their correlation was analyzed. Results： The expression of CSE, NF-lcB, and IL-8 mRNA in GES-1 cells in the H. pylori group was higher than that in the control group. The expression of CSE in the 200 tmaol/L NariS group and 400 utmol/L NariS group was lower than that of the control group （P〈0.05）, whereas the expression of NF-~cB and IL-8 in all NariS groups had no statistical differences compared with the control group （P〉0.05）. qqae expression of CSE, NF-r,B, and IL-8 mRNA in all groups of NariS, H. pylori ＋ 200 utmol/L NariS group, and H. pylori ＋ 400 [xrnol/L NariS group was lower than that in the H. pylori group （P〈0.05）. There was positive correlation among the expressions of CSE, NF-kB, and IL-8 mRNA in the H. pylori group, the H. pylori ＋ 200 utmol/L NariS group, and the H. pylori ＋ 400 uunol/L NariS group （P〈0.05）. Conclusion： H. pylori can induce NF-r,B and IL-8 mRNA expression and upregulate CSE mRNA expression. At 200 and 400 [unol/L, NariS can suppress H. pylori-induced NF-KB and IL-8 mRNA expression and ameliorate the morphology of H. pylori-induced GES-1 iniur~ which may protect gastric epithelial cells by H. pylori infection.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2013年第10期977-983,共7页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(81172301)
湖南省自然科学基金(S2010J5043)~~
