摘要
目的了解腺苷脱氨酶(ADA)、α-L-岩藻糖苷酶(AFU)、胆碱脂酶、前清蛋白和5′-核苷酸酶(5′-NT)在肝脏疾病诊断中的意义。方法选择332例肝脏疾病患者,其中,急性肝炎150例,重型肝炎25例,肝硬化40例,酒精性肝炎35例,慢性肝炎82例;选择同期90例健康体检者作为对照。采用Bayer ADVIA 2400全自动生化分析仪检测其血清ADA、AFU、前清蛋白、胆碱脂酶和5′-NT,绘制受试者工作特征曲线(ROC)并计算曲线下面积(AUC),评价5项指标在不同肝脏疾病中的诊断意义。结果重型肝炎、肝硬化患者血清ADA、AFU、5′-NT显著增高,前清蛋白、胆碱脂酶显著降低;ADA、AFU、5′-NT的异常值比例分别超过80%、65%、90%。急性肝炎、酒精性肝炎患者血清ADA、AFU、5′-NT轻度升高,前清蛋白、胆碱脂酶轻度下降;急性肝炎患者血清胆碱脂酶、5′-NT的异常值比例分别为80.0%、54.67%。血清前清蛋白、胆碱脂酶的AUC在重型肝炎患者中分别为0.022、0.000,在肝硬化组患者中分别为0.019、0.000;上述两组患者血清ADA、5′-NT的AUC均大于0.950,AFU的AU均大于0.800。在急性肝炎和酒精性肝炎组中,患者血清ADA、5′-NT的AUC均大于0.800,AFU的AUC分别为0.814、0.637。332例患者血清前清蛋白、胆碱脂酶的AUC分别为0.175、0.181,血清ADA、AFU、5′-NT的AUC分别为0.850、0.743、0.714。结论血清ADA、AFU、前清蛋白、胆碱脂酶和5′-NT检测对肝脏疾病的诊断具有重要价值。
Objective To understand the significance of adenosine deaminase（ADA）,alpha-L-fucosidase（AFU）,cholinesterase, prealbumin and 5＇-nucleotidase（SCNT） in diagnosis of liver diseases. Methods 332 patients with liver disease were selected. A mong them, there were 150 cases of acute hepatitis, 25 severe hepatitis,40 liver cirrhosis, 35 alcoholic hepatitis and 82 chronic hepatitis. Another 90 healthy people were selected as the control in the corresponding period. Bayer ADVIA 2400 automatic biochemical analyzer were adopted to detect their serum ADA, AFU, cholinesterase, prealbumin and 5＇-NT. Receiver operator characteristic eurve（R（DC） was drawn and the area under the eurve（AUC） were calculated. The diagnostic significance of the five indicators above was evaluated for different liver diseases. Results Serum ADA, AFU, 5＇- NT levels of patients with severe hepatitis and liver cirrhosis increased markedly,while prealbumin and cholinesterase decreased obviously, and the abnormal value percentages of ADA, AFU, 5＇-NT exceeded 80 %,65 % and 90%, respectively. Serum ADA, AFU, 5＇- NT levels of patients with acute hepatitis and alcoholic hepatitis increased slightly, while prealbumin and cholinesterase decreased mildly. The abnormal value percentages of serum cholinesterase and 5＇-NT in patients with acute hepatitis were 80.0% and 54.67 %, respectively. In patients with severe hepatitis, the AUC of serum prealbumin and cholinesterase were 0. 022 and 0. 000, respectively, and those in patients with liver cirrhosis were 0. 019 and 0. 000,respectively. In patients of two groups above,the AUC of serum ADA and 5CNT were all greater than 0. 950,and those of serum AFU were all greater than 0. 800. In acute hepatitis and alcoholic hepatitis group,the AUC of serum ADA and 5＇-NT were all greater than 0. 800,and those of AFU were 0. 814 and 0. 637,respectively. The AUC of serum prealbumin and cholin esterase of 332 patients were 0. 175 and 0. 181, respectively,and those of serum ADA, AFU,5%NT were 0. 850,
出处
《国际检验医学杂志》
CAS
2013年第21期2832-2834,共3页
International Journal of Laboratory Medicine
