摘要
目的:优选复方必清片的提取工艺。方法:在单因素试验基础上,以薯蓣皂苷元、秋水仙碱含量和浸膏得率的综合评分为指标,通过正交试验优选菝葜等7味药材的乙醇提取工艺;以总多酚含量和浸膏得率为综合评价指标,通过正交试验优选余甘子等5味药材的水提取工艺。采用HPLC测定薯蓣皂苷元和秋水仙碱的含量,色谱条件为Cosmosil C18色谱柱(4.6mm×250 mm,5μm),柱温35℃,流速1.0 mL·min-1,进样量10μL,检测波长分别为203,350 nm,流动相分别为甲醇-水(90∶10),甲醇-水(37∶63);采用紫外分光光度法测定总多酚含量。结果:菝葜等7味药材的最佳提取工艺为加10倍量70%乙醇提取2次,每次1.0 h;余甘子等5味药材的最佳提取工艺为加10倍量水提取3次,每次1.0 h。结论:优选的提取工艺稳定可行,为复方必清片的质量控制提供实验依据。
Objective： To optimize extraction technology of compound Biqing tablets. Method： Based on single factor tests, orthogonal design was adopted to optimize ethanol extraction technology of Smilacis Chinae Rhizoma and other six herbs with composite score of diosgenin content, colchicine content and extract yield as index; With extract yield and the content of total polyphenols as comprehensive evaluation index, water extraction technology of Phyllanthi Fructus and other five herbs was optimized by orthogonal test. The contents of diosgenin and colchicine were determined by HPLC, chromatographic conditions were as follows： Cosmosil C,s column （4. 6 mm 250 mm, 5 μm）, column temperature 35 ℃, flow rate 1.0 mL ＂min-1, injection volume 10 μL, detection wavelength at 203, 350 nm, mobile phase with methanol-water （90 ： 10） and methanol-water （37： 63） ; The content of total polyphenols was determined by UV. Result： Optimum ethanol extraction technology was as following： extracted 2 times with 10 times the amount of 70% ethanol, 1 h per time; Optimum water extraction technology was： extracted 3 times with 10 times the amount of water, 1 h each time. Conclusion： This optimized technology was stable and feasible, it could provide an experimental basis for quality control of compound Biqing tablets.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期51-55,共5页
Chinese Journal of Experimental Traditional Medical Formulae
