摘要
目的:研究海带多糖诱导血管平滑肌细胞凋亡及其调控机制。方法:在碱性成纤维细胞生长因子(bFGF)诱导血管平滑肌细胞增殖模型基础上,运用MTT法考察海带多糖对血管平滑肌细胞(VSMC)增殖的抑制作用;利用荧光倒置显微镜观察VSMC细胞形态学的变化;通过流式细胞术分析VSMC细胞周期的分布;采用Western blotting免疫印迹法探讨VSMC细胞凋亡相关蛋白Bax和Bcl-2的表达。结果:海带多糖抑制VSMC细胞增殖抑制。细胞形态学观察结果及流式、Western blotting免疫印迹分析结果表明海带多糖可诱导VSMC细胞凋亡。其凋亡机制与其诱导细胞周期阻滞于S期以及提高Bax/Bcl-2比率有关。结论:海带多糖可诱导VSMC细胞凋亡。
Purpose： To study the mechanism of apoptosis induced by Laminaria japonica polysaccharide. Methods： The proliferation of vascular smooth muscle cells induced by basic fibroblast growth factor （bFGF） was evaluated using MTT assay; The morphological changes of vascular smooth muscle cells were investigated by fluorescence microscope; The cell cycle distribution of vascular smooth muscle cells was determined using flow cytometry; The apoptotic factors were evaluated by Western-blot assay. Results： Laminaria japonica polysaccharide exhibited anti-proliferative activity on vascular smooth muscle cells in a dose-dependent manner. Further investigation indicated that Laminaria japonica polysaccharide induced vascular smooth muscle cells apoptosis. The apoptotic mechanism was related to the induction of S phase arrest, as well as regulating Bcl-2 family proteins expressions. Conclusions： Laminaria japonica polysaccharide could induce apoptosis.
出处
《食品科学》
CAS
CSCD
北大核心
2013年第21期344-348,共5页
Food Science
基金
福州大学科研启动基金项目(2010-XY-16)
福建省科技重大专项(2010NZ0001-1)
