摘要
设计并应用新的高效筛选程序 ,从植物根际土壤分离出同时具有促进植物生长和抑制黄瓜枯萎病害的假单胞菌株 M1 8.它对黄瓜幼苗的根系具有明显的促进作用 ,在培养皿中 ,M1 8能有效地抑制黄瓜枯萎病菌以及多种其他植物病原菌菌丝体的生长 .经 M1 8活菌浸种处理春黄瓜种子并在大棚内浇灌定植后的根际土壤 ,与对照相比 ,黄瓜枯萎病的株发病率降低 70 %～ 80 % ,霜霉病的发病率和病情指数都同时平均下降 70 %左右 ,黄瓜产量提高 2 0 %以上 .M1 8的生物防治效果达到极显著程度 .对培养液中纯化的抗真菌物质进行光谱分析和 LC- MC测定的结果表明 ,M1
A novel high efficiency procedure was designed and adopted for screening bifunctional Pseudomonas sp .M18. This strain has 1 aminocyclopropane 1 carboxylate(ACC)deaminase activity and was considered to be a plant growth promoting rhizobacterium.And it was demonstrated to has strong suppression activity against Fusarium spp ., as well as several other fungal phytopathogens.In the following field trial under commercial greenhouse condition, cucumber seeds were treated with M18 suspension,then the cucumber rhizosphere was treated with 1/100 diluted suspension after transplanting. It was found that M18 could preferentially colonize 10 times more in the rhizosphere than in the soil of surface layer. The systemic disease resistance was induced in the M18 protected cucumber plant.Compared with CK group,average incidence of wilt disease dropped down 70%～80%,and disease index of Downy Mildew also reduced by 70%.As a result,the cucumber yield was increased for 24% more. The biological control efficiency reached a very significant level according to the test. The antifungal compound was extracted and purified by silica thin layer chuomatography and C18 reversed HPLC and it was identified as phenazine 1 carboxylic by UV,IR,LC MS spectrum analysis.
出处
《上海交通大学学报》
EI
CAS
CSCD
北大核心
2000年第11期1574-1578,共5页
Journal of Shanghai Jiaotong University
基金
上海市科技发展基金项目! ( 9954130 2 8)
关键词
生物防治
假单胞菌株M18
羧基吩嗪
黄瓜枯萎病
biological control
Pseudomonas M18
ACC deaminase
phenazing 1 carboxylic acid
cucumber Fusarium wilt disease
