摘要
目的探讨原发性干燥综合征(primary Sjgren’s syndrome,pSS)患者外周血单个核细胞(PBMC)表面P2X7受体表达情况及其与焦虑、抑郁的相关性。方法用汉密尔顿焦虑、抑郁量表对31例pSS患者(pSS组)进行心理评估,分离其PBMC,在有或无ATP刺激后用流式细胞术检测CD14+PBMC及CD14-PBMC表面P2X7受体的表达水平,并用酶联免疫吸附试验测定ATP刺激前后白介素(interleukin,IL)-1β、IL-6水平。另外,分别设有19例类风湿关节炎(rheumatoid arthritis,RA)患者(RA组)及18例单纯焦虑、抑郁障碍患者(焦虑抑郁组)作为阳性对照,20例健康人(健康组)为阴性对照。结果所有4组受试者PBMC表面均有P2X7受体表达。ATP刺激较未刺激,CD14+PBMC(单核细胞)表面P2X7受体表达仅在pSS组[(5.6%3±2.48%)vs.(3.98%±1.68%),t=3.647,P=0.001]显著增高,而在CD14-PBMC(主要为淋巴细胞)表面其表达在pSS组[(16.92%±9.25%)vs.(10.06%±4.35%),t=4.908,P<0.001]及焦虑抑郁组[(12.68%±8.15%)vs.(8.07%±5.05%),t=3.412,P=0.003]均显著增高。ATP刺激后,pSS组CD14+PBMC表面P2X7受体表达显著高于RA组[(5.63%±2.48%)vs.(4.15%±2.20%),P=0.044]、焦虑、抑郁组[(5.63%±2.48%)vs.(3.35%±1.80%),P=0.004]及健康组[(5.63%±2.48%)vs.(3.25%±2.05%),P=0.002]。ATP刺激后,pSS患者PBMC上清中IL-1β水平与刺激后外周血CD14+PBMC表面P2X7受体表达呈显著正相关(r=0.447,P=0.025)。pSS组存在焦虑的患者占45.2%(1431),存在抑郁的患者占32.3%(1031)。ATP刺激后,CD14-PBMC表面P2X7受体表达与pSS组、焦虑抑郁组焦虑评分(r=0.344,P=0.030)及抑郁评分(r=0.319,P=0.045)呈显著正相关。结论 pSS患者PBMC表面P2X7受体高表达,可能与pSS的发病机制及其合并焦虑、抑郁相关。
Objective To study mononuclear cells （ PBMC ） the surface expression of P2X7 receptors （P2X7R） on peripheral in patients with primary Sjogren＇ s Syndrome （pSS） and its correlation anxiety and/or depression. Methods Hamilton Anxiety and Depression Scale （HAMA and HAMD） blood with wereused to assess 31 patients with primary Sjogren＇ s Syndrome. The expression of P2X7 receptors on the surface of CD14 ＋ PBMC and CD14 - PBMC with or without ATP stimulation was measured by flow cytometry （FCM）. The levels of IL-1 β and IL-6 in blood plasma and supernatants after ATP stimulation were measured by enzyme-linked immunosorbent assay （ELISA）. Nineteen patients with rheumatoid arthritis, 18 patients with anxiety and/or depression only were used as positive controls and 20 cases of healthy persons were used as negative controls. Results The expression of P2XTR could be detected in all subjects. Compared to no ATP stimulation, a significant up-regulation of P2XTR expression on CD14＋ PBMC was observed after ATP stimulation only in pSS group [ （ 5.6% 3 ±2.48% ） vs. （ 3.98% ± 1.68% ）, t = 3.647, P = 0. 001 ] ; while on CD14- PBMC, it was significantly up-regulated in both pSS group [ （ 16. 92% ± 9. 25% ） vs. （ 10. 06% ±4. 35% ）, t = 4. 908, P 〈 0. 001 ] and anxiety/depression group [ （ 12. 68%± 8.15% ） vs. （8.07% ±5.05% ）, t =3.412, P =0. 003]. After ATP stimulation, P2XTR expression on CD14＋PBMC in the pSS group was significantly higher than the RA group [ （5.63%±2.48% ）vs. （4. 15% ±2.20% ）, P = 0. 044 ], anxiety/depression group [ （5.63% ± 2. 48% ） vs. （ 3.35%± 1.80% ）, P = 0. 004 ] and healthy controls [（5.63% ±2.48%） vs. （3.25% ±2.05%）, P =0.002]; meanwhile, in the pSS group, P2XTR expression on the CD14＋ PBMC was significantly positively correlated to the supernatants levels of IL-113 （r =0. 447, P =0. 025）. There was 45.2% （ 14/31 ）patients with anxiety and 32. 3% （ 10/ 31）with depression in the pSS group. The exp
出处
《中华临床免疫和变态反应杂志》
2013年第3期217-223,F0003,共8页
Chinese Journal of Allergy & Clinical Immunology
基金
2013年浙江省中医药科学研究基金计划(A类)(2013ZA117)
关键词
干燥综合征
P2X7受体
焦虑
抑郁
Sjogren ＇ s syndrome
P2X7 receptor
anxiety
depression
