摘要
采用RT-PCR和RACE方法从鹤望兰蓝色花瓣中克隆到1个ANS基因(SrANS)。该cDNA全长1 389 bp(GenBank收录号KC484623),具有完整的开放阅读框(ORF),共1 083个碱基,编码361个氨基酸。与其他物种的氨基酸序列都有一定的同源性,其中与鸢尾的ANS同源性最高,达到74%。系统进化树分析显示,鹤望兰SrANS与红掌蛋白亲缘关系较近。保守结构域预测表明,该基因编码的蛋白具有典型的ANS蛋白功能结构域,其保守结构域中含有铁离子及2-O-酮戊二酸结合位点,属于2OG-FeII_Oxy双加氧酶超家族。应用半定量PCR分析表明,SrANS在蓝色花瓣中高表达,且在花发育的花蕾中期和始花期表达量最高。
In the study, the full-length cDNA sequence ofANS gene （SrANS） was cloned from blue petals of Stre- litzia reginae Banks using RT-PCR and RACE techniques. The cDNA sequence was 1 389 bp and included a whole open reading flame of 1 083 bp, encoding a polypeptide of 361 amino acids. The amino acid was highly conserved compared with other ANS homologues and shared up to 74% homology with ANS from Iris hollandica. Phylogenetic analysis indicated that SrANS was more related to ANS from Anthurium andraeanum. The conserved structural domain analysis revealed that SrANS had the typical functional domains of ANS protein, containing 2-oxoglutarate and iron ion combination sites and belonging to the 2OG-Fe（II）-dependent dioxygenase superfamily. The semi-quantitative PCR analysis indicated that SrANS was highly expressed in mid-bud period and early flowering period, and the transcript level was highest in blue petals.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2013年第11期1620-1625,共6页
Chinese Journal of Cell Biology
基金
福建省重大专项专题项目(批准号:2010NZ0003)
福建省农业科学院科技创新团队重点科研项目(批准号:CXTD2011-20)资助的课题~~
关键词
鹤望兰
花青素合成酶
类黄酮生物合成
基因克隆
Strelitzia reginae Banks
anthocyanidin synthase
flavoniod biosynthesis
gene cloning
