摘要
探讨内皮素 1预处理和缺血预处理两种预处理方式与G蛋白有关的信号转导途径的异同。用 0 5nmol/ml内皮素 1左心室注射或夹闭左冠状动脉 5min/再灌 5min× 2进行预处理 ,然后两组均缺血 6 0min ,再灌 30min。观察心电变化 ,免疫印迹法测定心脏Gαq/11和Giα2的含量。结果显示 ,无论是内皮素 1预处理还是缺血预处理均明显减轻缺血再灌注性室性心律失常。与对照组相比 ,缺血预处理组Gαp/11含量升 77 8% (P <0 0 5 ) ,Giα2含量无明显改变。内皮素 1预处理组Gαq/11含量升高 110 6 % (P <0 0 1) ,Giα2含量下降 31 0 % (P <0 0 5 )。本研究结果提示 ,激活Gαq/11蛋白是两种预处理对心肌产生保护作用的共同信号转导通路 ,而Giα2蛋白在两种预处理中的作用方式有所不同。
The present study was undertaken to explore the mechanism of G protein mediated signal transduction pathway during endothelin 1 (ET 1) pre treatment and ischemic preconditioning (IP). Rats were divided into four groups: ET 1, IP, ischaemia reperfusion (IR) and control groups. ET 1 pre treatment model was prepared by administrating 0 5 nmol/(L·kg) ET 1 into rat left ventricle, whereas IP model was prepared by ligating the left coronary artery for 5 min followed by 30 min reperfusion. All the animals were subjected to 60 min regional ischaemia and 30 min reperfusion alternately and then parameters of ventricular arrhythmia and expression of cardiac Gαq/11 and Giα2 were measured. The results showed that the scores of ventricular arrhythmia decreased significantly in both ET 1 and IP treated groups as compared with IR group. In comparison with control group, Gαq/11 increased by 77 8% ( P <0 05) and 110 6% ( P <0 01) in IP and ET 1 group respectively. Giα2 showed no significant difference in IP group, while it decreased by 31 0% ( P <0 01) in ET 1 group. In conclusion, activation of Gαq/11 may be related to the protecting mechanism of ET 1 pre treatment and IP, whereas Giα2 may only play a role in ET 1 pre treatment. [WT5HZ]
出处
《生理学报》
CAS
CSCD
北大核心
2000年第6期459-462,共4页
Acta Physiologica Sinica
基金
SupportedbythefoundationofBeijingMunicipalHealthBureau
