摘要
目的 应用高分辨魔角旋转磁共振（HR-MAS NMR）波谱分析巴马小型猪阻塞性慢性胰腺炎（CP）早期的代谢变化特征.方法 39头巴马小型猪按随机数字表法分为对照组和CP组.采用主胰管近端不全结扎法制备CP模型,对照组仅开腹翻动胰腺及肠道后关腹.造模后第4、8、12周分批处死动物,获取胰腺组织.根据胰腺纤维化程度,将CP分为轻、中、重度.应用HR-MAS NMR波谱分析轻度CP组和对照组胰腺组织的代谢特征.结果 制模成功的24头巴马小型猪的胰腺组织经病理检查为轻度10头,中度5头,重度7头.经HR-MAS NMR波谱分析,轻度CP组胰腺组织的代谢产物中胆碱、甘氨酸、甜菜碱、乳酸、脂肪酸、磷酸胆碱/甘油磷酸胆碱峰值与对照组有差异,其中胆碱波峰显著增高,差异有统计学意义（5.02 ±1.96比1.56 ±0.79,P＜0.01）,而其他5种代谢产物峰的差异均无统计学意义.结论 与对照组比较,巴马小型猪轻度CP胰腺组织的胆碱波峰强度显著增高,这可能是区分正常和早期CP的标志.
Objective To investigate the metabolic characteristics of early stage of obstructive chronic pancreatitis （CP） in swine by high-resolution magic angle spinning nuclear magnetic resonance （HR-MAS NMR） spectroscopy.Methods A total of 39 Bama miniature swine were randomly divided into CP group and control group.The pancreatic main duct （PMD） of the swine in the experimental group underwent partial proximal ligation procedure.The swine in the control group underwent opening the abdomen and rubbing the pancreas and intestine.At the 4th,8th and 12th week after the operation,the swine were sacrificed to harvest the pancreas tissue for pathologically examination,which were classified as mild,moderate and severe according to the degree of fibrosis.The metabolic profiles from the pancreas of normal swine and mild CP swine were examined by using HR-MAS NMR spectroscopy.Results CP model was successfully established in 24 Bama miniature swine,and pathologically examination showed 10 was mild,5 was moderate and 7 was severe.Among the metabolites in mild CP,HR-MAS NMR analysis suggested that the peak of choline,glycine,betaine,lactate,fatty acid,phosphorylcholine/choline glycerophosphate was different with those in normal pancreas,and only the difference of choline was statistically significant （5.02 ± 1.96 vs 1.56 ± 0.79,P 〈 0.O1）,while the difference of other 5 metabolites was not statistically significant.Conclusions Compared with control group,mild CP in Bama miniature swine shows significantly increased choline,and this may be a marker for discrimination of normal and early CP.
出处
《中华胰腺病杂志》
CAS
2013年第5期320-323,共4页
Chinese Journal of Pancreatology
基金
国家自然科学基金，上海市科委基金，中国博士后科学基金，上海市领军人才队伍建设专项资金
关键词
胰腺炎
慢性
模型
动物
磁共振波谱学
代谢
Pancreatitis, chronic
Models, animal
Magnetic resonance spectroscopy
Metabolism
