摘要
目的运用快速液相-高分辨飞行时间质谱(RRLC-TOF/MS)技术鉴别淫羊藿大鼠给药后的入血成分及代谢产物,以探索淫羊藿可能的药效成分及体内代谢过程。方法色谱分离采用SHISEIDO MG C18(3.0mm×100mm,3μm)色谱柱;流动相为乙腈(A相)和0.1%甲酸水溶液(B相),梯度洗脱:0～3min(5%～19%A),3～13min(19%～32%A),13～25min(32%～52%A),25～30min(52%～70%A);柱温25℃;流速0.6mL/min,柱后分流比为31。质谱定性采用飞行时间质谱,电喷雾离子源(ESI),正离子模式,质量数扫描范围m/z 100～1 000。结果鉴别出淫羊藿中3种入血成分及4种代谢物。结论所建方法快捷、有效,可用于淫羊藿体内成分鉴别,为进一步的药效试验提供了参考和依据。
Objective To identify the constituents and metabolites of epimedium in rat plasma by rapid-resolution liquid chromatography-time of flight mass spectrometry （RRLC-TOF/MS） after intragastric administration, so as to explore the active ingredients and metabolites of epimedium in vivo. Methods The separation was performed on a SHISEIDO MG C18 reverse phase column （3.0 mm×100 mm,3 μm）.The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute.The flow rate was 0.6 mL/min, post-column split ratio was 31, and the temperature of column was 25℃. Time-of-flight mass spectrometer（TOF/MS） and electrospray ion source （ESI） were applied for qualitative analysis under positive ion mode, with the mass scan range being m/z 100-1 000. Results Three prototype constituents and 4 metabolites of epimedium were identified in rat plasma. Conclusion A rapid and efficient RRLC-TOF/MS method has been established for studying the constituents and metabolites of epimedium in vivo, which provides a reference for further pharmacodynamics experiments.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2013年第10期1104-1107,共4页
Academic Journal of Second Military Medical University
关键词
淫羊藿
入血成分
代谢产物
飞行时间质谱
Epimedium brevicornum
prototype constituents metabolites time of flight/mass spectrometry
