摘要
目的观察本实验室研制的甲型肝炎灭活疫苗的安全性和免疫原性。方法甲型肝炎病毒吕－８ 株在人胚肺二倍体细胞（ＫＭＢ１７）中增殖，收获的病毒液用福尔马林灭活制成实验性疫苗，接种普通狨猴。结果有 特异性抗ＨＡＶ产生，无血清酶活性升高和肝组织病理学改变。接种疫苗的狨猴均能抵抗甲肝病毒强毒株的攻击， 而对照组均出现酶活性升高和肝组织病理学改变。结论该疫苗具有良好的免疫原性和可靠的安全性。
Objective To study on the safety and immunogenicity of inactivated hepatitis A vaccine in common marmosets(Callithris jacchus). Methods After the Lu-8 strain of hepatitis A virus was proliferated in human fetal lung diploid cells(KMB17), the harvested virus was inactivated with formalin and used for inoculating marmosets as an experimental vaccine. Results Specific anti-HAV were induced in the marmosets, and no elevation of serum enzyme activity or pathological exchange of liver tissue were observed. The vaccine protected marmosets against the intravenous challenge with virulent HAV. However, the elevation of serum enz- yme activity and pathological exchange of liver tissue were observed in all the control marmosets. Conclusion The experimental inactivated HA vaccine has good immunogenicity and safety.
出处
《中国生物制品学杂志》
CAS
CSCD
2000年第1期33-35,共3页
Chinese Journal of Biologicals
关键词
甲肝灭活疫苗
普通狨猴
安全性
免疫原性
Inactiyated hepatitis A vaccine Common marmoset Safety Immunogenicity
