摘要
采用凯氏定氮法、苯酚-硫酸比色法和双波长比色法测定唐古特大黄种子、掌叶大黄种子和药用大黄种子的蛋白质、多糖和淀粉的含量。采用凯氏定氮法测定蛋白质含量,苯酚-硫酸比色法测定多糖含量,双波长比色法测定淀粉含量。唐古特大黄种子的蛋白质和多糖在三种大黄种子中含量最高,分别为21.81%、6.58%,掌叶大黄种子淀粉含量在三种大黄种子中最高,含量为32.95%。三种大黄种子中,蛋白质含量为:唐古特大黄种子>掌叶大黄种子>药用大黄种子;多糖含量为:唐古特大黄种子>掌叶大黄种子>药用大黄种子;淀粉含量为:掌叶大黄种子>唐古特大黄种子>药用大黄种子。大黄种子的蛋白质、多糖、淀粉含量丰富。测定方法精密度、准确度、稳定性均符合生物样品测定要求,可用于大黄种子蛋白质、多糖和淀粉含量的测定。
The aim was to carry out a study about the concentrations of protein,polysaccharides, starch detected in the Rhubard seeds. The contents of protein were determined by kjeldahl method.The contems of polysaccharides were determined by phenol-vitriol colorimetry method. The contents of starch were determined by dual-wavelength colorimetric method. The results showed that Rheum. tanguticum seeds proteins and polysaccharides in the three authentic rhubarb seeds were 21.81%, 6.58% ,Rheum palmatum seeds starch content in the three authentic rhubarb seeds were 32.95M. Protein content of three authenIic rhubarb seeds were：Rheum, tanguticum seeds Rheum palmatum seeds ） Rh. officinale seeds; polysaccharide content were： Rheum. tanguticum seeds ）Rheum palmatum seeds 〉 Rh. officinale seeds; starch content were. Rheum palmatum seeds 〉 Rheum tanguticum seeds〉 Rh. officinale seeds. Rhubarb seeds contain abundant protein, polysaccharides; starch. The methods is proved to be accurate, precise, stable and suitable for the protein, polysaccharides and starch in Rhubarb seeds.
出处
《光谱实验室》
CAS
2013年第6期3114-3121,共8页
Chinese Journal of Spectroscopy Laboratory
基金
国家科技支撑计划项目(2011BA105B03)
关键词
大黄种子
蛋白质
多糖
淀粉
Rhubarb Seeds
Protein
Polysaccharides
Starch
