摘要
目的:建立大鼠血浆中棉酚含量的高效液相色谱测定方法。方法:采用ODS-C18色谱柱(4.6 mm×200 mm,5μm),柱温25℃,流动相为甲醇-1%磷酸溶液(85∶15),流速1.0 ml/min,检测波长:235 nm。结果:在0.33～3.30μg/ml范围内峰面积与浓度呈现良好的线性关系(r=0.999 4),检测限为0.04μg/ml;平均回收率为94.7%～97.3%;给药组大鼠血浆中棉酚的含量为0.68～2.14μg/ml。结论:该方法可用于棉酚生物样品分析。
Objective ：To establish an HPLC method for determination of gossypol in rat plasma. Methods ：An ODS -C18 column （4.6 mm ×200 mm, 5 μm） was used as stationary phase, and methanol and 1% phosphoric acid solution （85： 15, v/v） as mobile phase with the flow rate of 1.0 ml/min. The detective wavelength was at 235 nm. Results ：The peak area of gossypol and its concentration showed a good linear relationship when the concentrations varied from 0.33 - 3.30 μg/ml, related coefficient being 0. 999 4. The limit of detection was 0.04μg/ml （ S/N = 3 ）. The relative recovery was between 94.7% and 97.3%. The content of gossypol in rat plasma was between 0.68 μg/ml and 2.14μg/ml. Conclusion：This method can be used for analysis of gossypol in biological samples.
出处
《天津药学》
2013年第5期1-3,共3页
Tianjin Pharmacy
基金
新疆维吾尔自治区高校科研计划青年教师科研启动基金项目(No.XJYDU2007S21)
