摘要
为研究复合益生菌对高热环境中断奶仔猪生长性能、肠道菌群和热休克蛋白表达的影响,将复合益生菌分别以1%、3%的水平添加到基础日粮中组成试验日粮1和2,同时以基础日粮作为对照。选取45头35日龄杜洛克断奶仔猪,随机分为3组,分别饲喂上述日粮,进行为期42 d的饲养试验。结果表明,在高热环境中,3%复合益生菌试验组仔猪日增重显著高于对照组(P<0.05),料重比显著低于对照组(P<0.05);2个试验组仔猪肠道中的乳酸杆菌数比对照组显著增高(P<0.05),而大肠杆菌数与腹泻率则显著降低(P<0.05)。与对照组相比,1%复合益生菌对Hsp70和Hsp27 mRNA水平无显著影响,而3%复合益生菌可显著降低Hsp70和Hsp27 mRNA水平。上述结果说明,复合益生菌能有效缓解断奶仔猪的热应激,提高断奶仔猪的生长性能,改善肠道菌群,显著降低腹泻率,可作为一种潜在的仔猪抗热应激添加剂。
The growth performance, intestinal microbiota and expression of heat shock protein of weaned piglets af- fected by multi-probiotics under hyperthermic conditions were studied. The muhi-probiotics was supplemented into a basal diet at 0 （control）, 1%, and 3% levels. 45 weaned Duroc piglets aged 35 d were selected and divided into 3 groups ran- domly. The feeding last for 42 d. Under hyperthermic conditions, the daily weight gain of piglets in 3% supplemental group was significantly higher than that of control （ P〈0. 05 ） , and feed conversion ratio was significantly lower （ P〈0. 05 ）. Theintestinal lactobacillus counts of the two experimental groups were both significantly higher than that of control （P〈0. 05 ） , while the number of Escherichia. coli and di- arrhea incidence were significantly lower （ P 〈 0. 05 ）. Compared with the control, adding 3% muhi-probiotics dramaticlly lowered the mRNA expressions of HspTO and Hsp27 which were not affected by 1% muhi-probiotis sup- plement. However, these results indicated that multi-pro-biotics relieved heat stress, improved growth performance and intestinal microbiota, and reduced diarrhea incidence of weaned piglets. It could be used as a potential additives for piglets in respouse to heat stress.
出处
《江苏农业学报》
CSCD
北大核心
2013年第5期1070-1074,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31272627)
公益性行业(农业)科研专项经费(201003060)
2012江苏省挂县强农富民工程金陵科技学院专项
关键词
复合益生菌
仔猪
高热环境
生长性能
肠道菌群
热休克蛋白
multi-probiotics
piglet
hyperthermic condition
growth performance
intestinal microbiota
heatshock protein
