摘要
目的建立HPLC法测定麝香接骨胶囊中伪麻黄碱、麻黄碱、马钱子碱和士的宁的含量。方法色谱柱:SHIMADZU VP-ODS C18柱(150 mm×4.6 mm,5μm);流动相:乙腈(A)-0.01 mol·L-1庚烷磺酸钠与0.02 mol·L-1磷酸二氢钾等量混合溶液(B)(用10%磷酸调节pH值2.8)(19∶81);检测波长:206 nm;流速:1.0 ml·min-1;柱温:35℃。结果伪麻黄碱在1.51～48.4 ng、麻黄碱在16.09～515 ng、马钱子碱在12.67～405 ng、士的宁在16.29～521.25 ng范围内线性关系良好;平均回收率分别为伪麻黄碱96.87%(RSD为2.21%)、麻黄碱99.12%(RSD为2.69%)、马钱子碱101.66%(RSD为2.85%)、士的宁96.35%(RSD为2.74%),线性范围分别为。结论此方法简便,准确,灵敏,可用于麝香接骨胶囊中麻黄和马钱子的质量控制。
Objective To develop a method for the determination of ephedrine, pseudoephedrine, brucine and strychnine in Shexiang Jiegu capsules. Methods SHIMADZU VP-ODS C18 （150 mm × 4. 6 mm, 5 μm） was used. The mixed solution（pH was adjusted to 2. 8 with phosphate acid）composed of 0. 01 mol ·L^-1 sodium-heptane- sulfonate solution and an equal quantity of 0. 02 mol·L^-1 potassium dihydrgen phosphate solution was chosen as mobile phase B while acetonitrile was used as mobile phase A to elute in a gradient program of A-B（ 19： 81 ） . The detection wavelength was 206 nm and the flow rate was 1. 0 ml ？min ^- 1, with the column temperature at 35℃ . Re-sults Good linear relationships were obtained within the ranges of 1. 51 -48. 4 ng for pseudoephedrine,16. 09 -515 ng for ephedrine, 12. 67 -405 ng for brucine and 16. 29 -521. 25 ng for strychinine. The average recoveries（ n = 6） were 96. 87% （ RSD =2. 21% ） for pseudoephedrine,99. 12% （RSD =2. 69% ） for ephedrine, 101. 66% （ RSD =2. 85% ） for brucine,and 96. 35% （ RSD - 2. 74% ） for strychnine. Conclusion This method is simple, accurate and sensitive. It can be used for quality control of herba ephedrae and semen strychni in Shexiang Jiegu capsules.
出处
《解放军药学学报》
CAS
2013年第5期450-452,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
