摘要
目的探讨青海省鼠疫菌株生物学特点及流行病学意义，为鼠疫防治提供科学依据。方法对2003～2012年青海省分离的鼠疫菌株进行生化及糖醇类酵解试验、毒力因子检查和毒力测定。结果根据生化分型指标，131株被试菌株中120株为青藏高原型，3株为祁连山型，其余8株菌与青海省疫源地生态型菌株均不相同。被试100株代表菌株均能产F1抗原和PstI，均具有Vw抗原因子；Pgm-菌株占95．00％（95／100），Pgm±菌株占4．00％（4／100），Pgm-菌株占1．00％（1/00）。毒力测定结果显示，最小致死量（MLD）均在10000个菌以下，均为强毒株。结论青海高原喜马掩雅旱獭鼠疫自然疫源地2003～2012年分离的鼠疫菌具备青藏高原鼠疫病原体特性，鼠疫菌的毒力强，对人的健康威胁严重。
Objective To investigate the biological characteristics and epidemiological significance of Yersinia pestis strains in Qinghai Province in order to provide a scientific basis for plague prevention and control. Methods Biochemical typing and glycolysis testing were performed on Y. pestis isolated in Qinghai Province from 2003--2012, and its virulence factors and virulence were determined. Results According to biochemical typing, 120 of the 131 strains tested were from the Qinghai-Tibet Plateau, 3 were from the Qilian Mountains, and the remaining 8 were different ecotypes from plague loci on the Qingzang plateau. One hundred strains of Y. pestis expressed pesticin I （PstI＋ ）, had an elaborate en velope or fraction antigen （Fra＋）, and had the ability to produce V and W antigens （Vwa＋ ）. Of those strains, 95N formed red colonies （pigmented or Pgm＋ ）, 1% formed white colonies （Pgm）, and 4% formed white and red colonies （Pgm＋- ）. Virulence testing indicated that the minimum lethal dose was fewer than 10,000 bacteria, and all of the Y, pestis strains were highly virulent. Conclusion The Y. pestis strains isolated from natural loci of plague transmitted by Himalayan marmots on the Qinghai Plateau had characteristics of plague from the Qinghai Tibet Plateau. Y. pestis is highly virulent and represents an extremely serious threat to human health.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第9期821-823,共3页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.81160211)
关键词
青海省
鼠疫
病原学
流行病学
Qinghai Province, plague
etiology
epidemiology
