摘要
为了筛选能够高效净化水质的光合细菌菌株,采用半固体试管法和双层平板法,从市售光合菌菌液中分离得到2株光合细菌,分别命名为GHJ-1、GHJ-2,对菌株进行了形态学观察、生理生化及16S rDNA鉴定,比较了这2株光合细菌对青鱼(Mylopharyngodon piceus)养殖污水的净化效果。结果表明:经鉴定这两株光合细菌为Rhodovulum sp菌株。菌株GHJ-1、GHJ-2对污水进行15 d的净化处理后,硝酸盐去除率分别为91.4%和96.3%;亚硝酸盐的去除率分别为68.9%和82.7%;氨氮去除率分别为56.8%和67.5%;COD Mn的去除率分别为61.7%和42.4%。
In order to select the photosynthetic bacteria （PSB） strains to be used for aquaculture wastewater purification, two PSB strains, named GHJ-1, GHJ-2, were isolated from commercial PSB broth by anaerobic culture, semi-solid tube and double-layer plate culture methods. The two obtained strains were investigated based on morphology, physio-biochemi- cal characteristics and 16S rDNA sequence analysis. Their purification effects for the effluent from pond of Mylopharyng- odon piceus culture were determined in the experiment. Both of the strains GHJ-1 and GHJ-2 were identified as Rhodovu- lure sp. The removal rates for nitrate, nitrite, ammonia nitrogen and chemical oxygen demand （COD） from the pond ef- fluent, by strains GHJ-1 and GHJ-2, were 91.4% and 96. 3% , 68.9% and 82. 7% , 56. 8% and 67.5% , 61.7% and 42.4% , respectively.
出处
《淡水渔业》
CSCD
北大核心
2013年第5期57-61,共5页
Freshwater Fisheries
基金
国家水体污染控制与治理科技重大专项(2011ZX07101-012-008)
现代农业产业技术体系建设专项资金(CARS-46-21)
浙江省重大科技专项重大农业项目(2010C02001)
浙江省重点科技创新团队项目(2012R10026-14)
湖州市重大科技专项(2010ZD1002)
湖州市自然科学资金项目(2010YZ08)
