摘要
目的探讨丁苯酞对氧糖剥夺/复氧的原代大鼠海马神经元磷酸化环磷酸腺苷反应原件结合蛋白(CREB)和Bcl-2表达的影响。方法以原代大鼠海马神经元设立对照组、模型组、丁苯酞组。丁苯酞组复氧8h后应用终浓度为0.1、1、10μmol/L的丁苯酞干预,通过Western blot法检测磷酸化CREB蛋白、RT-PCR法测定Bcl-2mRNA的表达。结果与对照组比较,模型组和不同浓度丁苯酞组大鼠海马神经元磷酸CREB蛋白、Bcl-2mRNA表达明显降低(P<0.05,P<0.01);与模型组比较,不同浓度丁苯酞组磷酸化CREB蛋白、Bcl-2mRNA表达明显升高,差异有统计学意义(P<0.01)。结论在实验剂量范围内,丁苯酞呈剂量依赖性地抑制海马神经元的凋亡,其作用机制可能是通过上调磷酸化CREB表达,提高Bcl-2活性,抑制海马神经元凋亡。
Objective To study the effect of dl-3-N-butylphalide (NBP) on expression of p-CREB and Bcl-2 in rat hippocarnpal neurons. Methods Rat primary hippocarnpal neurons were divided into control group, model group, and dl-3-NBP group. D1-3-NBP group was treated with 0. 1 μmol/L, 1 μmol/L and 10 μmol/L dl-3-NBP, respectively, 8 h after reoxygenation. Expression of p-CREB and Bcl-2 rnRNA was detected by Western blot and RT-PCR,respeetively. Results The expression level of p-CREB and Bcl-2 mRNA in hippocarnpal neurons was significantly lower in model group and dl-3-NBP group than in control group (P〈0.05, P〈0.01), and was significant- ly higher in dl-3-NBP group than in model group (P〈0.01). Conclusion D1-3-NBP inhibits ap- optosis of hippocarnpal neurons in a dose-dependent manner by upregulating the expression of p-CREB and the activity of Bcl-2.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2013年第10期1081-1083,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases