摘要
目的:探讨PTEN基因在食管鳞癌细胞中的表达水平及突变情况。方法:采用RT-PCR技术检测高分化的Eca109、低分化的EC9706和EC1食管鳞癌细胞株细胞中PTEN mRNA的表达水平,并克隆PTEN基因全长,与野生型PTEN基因序列比对,分析突变情况。结果:EC1、EC9706、Eca109细胞株中PTEN mRNA的表达水平分别为(0.06±0.02)、(0.24±0.02)、(0.41±0.01),3株细胞中PTEN mRNA的表达水平差异有统计学意义(F=306.330,P<0.001),Eca109细胞中PTEN mRNA的表达水平高于EC9706和EC1细胞(P<0.05)。3株细胞中PTEN基因均存在不同程度突变,突变主要集中在外显子5和8。结论:食管鳞癌细胞中PTEN表达水平与细胞的分化程度有关,PTEN基因突变与食管鳞癌的发生发展有关。
Aim：To study the expression level and mutation of PTEN gene in esophageal squamous cell carcinoma （ESCC） cells.Methods：The expression of PTEN mRNA in three ESCC cell lines （EC9706,EC1 of low differentiation and Eca109 of high differentiation ） was detected by RT-PCR.The full length of PTEN gene in the cell lines mentioned above was cloned , and then the sequences of PTEN gene were blasted with the sequence of wide type PTEN gene on GenBank to analyze the mutation sites.Results： The expression levels of PTEN in EC1, EC9706 and Eca109 cells were（0.06 ± 0.02）,（0.24 ±0.02） and （0.41 ±0.01）, and there were significant difference among the three cell lines （F=306.330, P 〈0.001）.That was higher in Eca109 cells with well differentiation degree than those in EC 9706 and EC1 cells with low differentiation degree（P〈0.05）.The mutations of PTEN gene in the three ESCC cell lines were found and the mutation sites mainly focused on exon 5 and 8.Conclusion： The expression level of PTEN has correlation with the differentiation degree and there are mutations of PTEN gene in ESCC .
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第5期577-580,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金项目3090178
关键词
食管鳞癌
PTEN基因
突变
esophageal squamous cell carcinoma
PTEN gene
mutation
