摘要
在我国东南沿海地区,每年均有人因食用带有河豚毒素的河豚鱼或织纹螺引起中毒的事件。本文以河豚毒素标准品溶液分析河豚毒素胶体金层析快速检测卡的最低检测出限为0.5 MU/mL或100 ng/mL。根据河豚毒素限量值(10 MU/g)设计了织纹螺以及河豚鱼的样品前处理方法,进一步分析了20个织纹螺及河豚鱼实际样品,准确率为100%,单个样品测试时间为20 min。结果表明:河豚毒素胶体金层析快速检测卡具有灵敏度高、特异性好、操作简便、可通过肉眼直接判读结果的特性,尤其适合于基层检测机构、渔业管理单位及水产品加工企业对河豚鱼或织纹螺中河豚毒素的快速筛查与分析。
Many people died for eating nocuous Nassarius or puffer fishes which contain neurotoxic tetrodotox- in in southeastern China. The detecting limits of immunochromatography test strip were about 0. 5 MU/mL or 100 ng/mL, which were confirmed by tetrodotoxin standard solution experiment. On the basis of limited quan- tity of tetrodotoxin, sample pre - treatment method was designed for detecting 20 samples including both Nas- sarius and puffer fishes. The precision rate of detecting assay reached 100% and single sample testing time was about 20 minutes. The results showed that the immunochromatography test strip had many characters of high sensitivity, good specificity, user- friendly control, reading results by the unaided eye, and etc. The testing strip can be applied to testing organization at grassroots level, fishery management grassroots units, and aquatic product process enterprise for tetrodotoxin screening in fishes and snails.
出处
《福建水产》
2013年第4期323-327,共5页
Journal of Fujian Fisheries
基金
福建省公益类科研院所基本科研专项[闽科计(2011)50号]
厦门市科技计划项目(项目编号:3502Z20122006)
关键词
河豚鱼
织纹螺
胶体金层析
河豚毒素筛查
puffer fishes
Nassarius
immunochromatography test strip
tetrodotoxin screening
