摘要
目的:探索姜黄素诱导宫颈癌细胞HeLa发生凋亡的效应和分子机制。方法:以不同浓度的姜黄素(10,20,30,40,50μmol.L-1)作用于体外培养的宫颈癌HeLa细胞24 h,应用基于Annexin V-FITC染色的流式细胞技术和AO/EB双染的荧光显微镜方法,检测不同浓度姜黄素诱导HeLa细胞凋亡的形态改变和凋亡率;应用免疫细胞化学方法,检测凋亡蛋白酶caspase-3和caspase-8的表达水平。结果:流式细胞检测结果显示,姜黄素可诱导HeLa细胞发生凋亡,并随着药物浓度升高细胞凋亡率增加;荧光显微镜观察发现,在姜黄素的作用下,HeLa细胞数量明显减少,呈现特征性的凋亡形态;免疫细胞化学检测显示,HeLa细胞的caspase-3和caspase-8的表达水平随姜黄素作用浓度的增加而升高,呈现剂量依赖性。结论:姜黄素诱导HeLa细胞发生凋亡可能是通过caspase依赖性凋亡途径实现的。
OBJECTIVE To investigate the apoptosis induced by curcumin in cervical cancer HeLa cell line and its mechanism in vitro. METHODS The HeLa cells were treated with 10, 20, 31）, 40 and 50 μmol.L-1 curcumin for 24 hours respectively. The apoptotic rate of HeLa cells induced by curcumin was detected by flow cytometry based on Annexin V-FITC/PI staining. Morphological changes of apoptosis based on AO/EB staining were observed with fluorescent microscope. The expression level of caspase-3 and caspase-8 were detected by inmauno-cytochemistry method. RESULTS Flow cytometry analysis showed that curcumin could induce the apoptosis of HeLa cells, the apoptotic rates were increased with the increased of curcumin concentration. The AO/EB staining showed that the total number of HeLa cells decreased with the increasing of curcumin concentration, and part of the cells appeared apoptotic morphological characteristics obviously. The immuno-histochemistry staining indicated that the expressions of caspase-3 and caspase-8 proteins increased in a curcumin concentration-dependent manner. CONCLUSION The HeLa cells apoptosis induced by curcumin could be achieved through caspase-dependent pathway.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第17期1385-1389,共5页
Chinese Journal of Hospital Pharmacy
基金
湖北省教育厅科学技术研究重点项目(编号:D20101808)
湖北省教育厅科学技术研究计划优秀中青年人才项目(编号:Q20121606)
湖北中医药大学校级科研课题中青年项目(编号:201212)
