摘要
以还原糖产量和酶解效率为考查指标,对酶负荷、底物浓度、酶解时间等条件进行优化研究,并讨论纤维素酶重吸附再利用方法的可行性。结果表明:100mL反应液中,酶解预处理反应的最优工艺为酶负荷100mg、底物浓度25mg/mL、酶解时间48h,此条件下还原糖产量为1459mg,酶解效率为52.52%。利用酶解液进行光合微生物产氢实验,7d累积产氢量最高为1011mL。用底物吸附法进行纤维素酶重吸附利用,得出纤维素酶可进行3次高效再利用,对减少酶用量、降低制氢成本有重要意义。
In photosynthetic hydrogen production process, enzymatic hydrolysis of ultra-corncob powder have a significant impact on the efficiency and cost of hydrogen production. The paper is mainly through experiments, taken reducing sugar yield and enzymatic hydrolysis efficiency as reference, to study on the optimization of the conditions of the enzyme dosage , substrate concentration, reaction time, and discuss the feasibility of cellulase readsorption method of recycling. The results showed that in 100mL reaction solution, the optimal process of enzymatic hydrolysis pretreatment for the enzyme dosage time 48h. Under this condition, the reducing sugar yield 100mg, substrate concentration of 25mg/mL, hydrolysis was 1459mg, and enzymatic efficiency of 52. 52%. The enzymatic hydrolyzate was used for photosynthetic hydrogen production, the 7d cumulative hydrogen to 101 l mL. Cellulase re-adsorption method realized 3 amount of enzyme, and reducing the cost of hydrogen times efficient recycling, which are important production. production up to reduce the
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2013年第9期1536-1541,共6页
Acta Energiae Solaris Sinica
基金
国家自然科学基金(50976029)
国家高技术研究发展(863)计划(2012AA051502)
关键词
光合产氢
重吸附方法
酶解效率
还原糖产量
超微玉米芯粉
photosynthetic hydrogen production
re-adsorption method
enzymatic hydrolysis efficiency
reducingsugar yield
ultra-corncob powder
