摘要
目的:研究分析燕窝样品中唾液酸的含量与品种的相关性,为燕窝质控提供参考。方法:HPLC测定燕窝样品中唾液酸的含量,以邻苯二胺盐酸盐为衍生化试剂,流动相乙腈-水(15∶85),流速1.0 mL·min-1,检测波长230 nm。结果:25种燕窝中水分含量范围在9.13%～17.58%,唾液酸的含量范围在3.59%～15.22%。经实验方法检测,银耳、猪皮等伪品的唾液酸含量为零。结论:该法简便、可靠、准确,可用于燕窝与银耳、猪皮等伪品区分的辅助手段;初步的数据显示,虽然白燕、血燕、黄燕中唾液酸的含量无显著性差异,但可为进一步的燕窝质量优劣研究提供参考。
Objective： To study and analyze the content of sialic acid （SA） among different edible bird＇ s nest （EBN） , so as to provide reference for EBN quality standard. Method.： Using HPLC to detect the content of SA O-pheny-lenediamine ·2HC1 is the derivatization reagent; the mobile phase consisted of acetonitrile-water （15： 85） ; the flow rate was 1.0 mL·min^-1. Result： The water content in 25 kinds of EBN ranged from 9.13% to 17.58% , the SA content in 25 kinds of EBN ranged from 3.62% to 15.23% respectively. The content of SA in tremella and pigskin was found zero while using this method. Conclusion： The method is simple, reliable and precise, which could be used to identify the EBN, tremella and pigskin. Althought there＇s no difference among white, red and yellow EBN, the data could provide support for the further study on the quality of EBN.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第19期64-67,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然基金面上项目(81173498)
