摘要
目的 观察人RUNT相关转录因子2（Runx2）和半乳糖凝集素3（Galectin-3）在胶质瘤细胞U251中的表达及其相互关系,抑制两者的表达对脑胶质瘤细胞株U251增殖和凋亡的影响.方法 使用RNA干扰技术处理U251细胞,应用逆转录·聚合酶链反应（RT-PCR）和Western blot法检测Runx2和Galectin-3在mRNA和蛋白水平的改变；应用噻唑蓝（MTT）方法和流式细胞仪检测细胞增殖活性和凋亡的变化.结果 使用Runx2小干扰RNA（siRNA）处理U251细胞后,Runx2 （0.260±0.074）和Galectin-3（0.170±0.065）在mRNA和蛋白水平的表达均下降（P＜0.01）,使用Galectin-3siRNA处理U251细胞后,Galectin-3在mRNA水平和蛋白水平的表达下降（P＜0.01）,但Runx2的表达无明显变化（P＞0.05）；抑制Runx2和Galectin-3的表达后,胶质瘤U251细胞的增殖活性下降（P＜0.01）,凋亡率增加（P＜0.01）.结论 Runx2、Galectin-3在胶质瘤细胞株U251中表达,且Runx2调控Galectin-3的表达,抑制两者的表达可抑制U251细胞的增殖,促进细胞凋亡,丽者可能参与了胶质瘤的发生发展.
Objective To explore the expression of human runt-related transcription factor 2 （Runx2） and Galectin-3 in glioma cell U251,and to elucidate the influnce of small interfering RNA （siRNA）-mediated inhibiting Runx2 and Galectin-3 gene on proliferation and apoptosis of glioma cell U251.Methods U251 cells were treated with siRNA,then the expression of Runx2 and Galectin-3 mRNA and protein was detected by using reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blotting respectively.The change in cell proliferation was studied by methyl thiazol tetrazolium （MTT） assay.Cell apoptosis was examined by using flow cytometry （FCM）.Results When U251 cells were treated with Runx2-tarted siRNA,the expression of Runx2 （0.260 ± 0.074） and Galectin-3 （0.170 ± 0.065） in mRNA and protein leveld was reduced brokendown,but when the U251 cells were treated with Galectin-3 siRNA,only Galectin-3 expression was decreased in mRNA and protein levels,and the expression of Runx2 had no significant change （P ＜ 0.01）.The decrease of Runx2 and Galectin-3 expression resulted in the decrease of U251 cell proliferation activity （P ＜0.01）.The apoptosis rate in Runx2,and Galectin-3 siRNA groups was increased （P ＜ 0.01）.Conclusion Runx2 and Galectin-3 are expressed in glioma cell U251,and Runx2 mediates the expression of Galectin-3.Down-regulation of both Runx2 and Galectin-3 can suppress proliferation activity and induce apoptosis of U251 cells.Both Runx2 and Galectin-3 may be implicated in tumorigenesis and tumor progression of glioma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第9期1821-1824,共4页
Chinese Journal of Experimental Surgery
