摘要
目的:探讨生肌象皮膏促进糖尿病大鼠溃疡创面愈合的免疫-炎症反应机制。方法:将Wistar大鼠随机分成4组,一组为正常对照组,其余3组采用链脲佐菌素(STZ)尾静脉注射制备糖尿病动物模型,4组动物均于背部打孔,造成溃疡模型。3组糖尿病溃疡大鼠按照血糖和体重分层随机分为糖尿病对照组、凡士林组、生肌象皮膏组。生肌象皮膏组外用生肌象皮膏纱条外敷,凡士林组用凡士林纱条外敷,正常对照组、糖尿病对照组均以生理盐水纱条外敷,观察血清白介素-2(IL-2)水平与创面组织肿瘤坏死因子-α(TNF-α)含量的变化。结果:生肌象皮膏组大鼠糖尿病溃疡创面的愈合速度加快,与糖尿病对照组比较于第7,14,21,30天有显著性差异;生肌象皮膏组大鼠血清IL-2水平与糖尿病对照组比较于第21,30天降低,有显著性差异,与凡士林组比较第21,30天降低,有显著性差异;生肌象皮膏组大鼠创面组织TNF-αmRNA表达水平与糖尿病对照组比较于第21,30天降低,有显著性差异,与凡士林组比较第21,30天降低,有显著性差异。结论:生肌象皮膏可以促进糖尿病大鼠溃疡创面的愈合与调节免疫-炎症反应密切相关。
Objective： To investigate the mechanisms of the immune-inflammatory response of the Shengji Xiangpi mastic（SM） promoting the ulcer healing in diabetic rats.Method： Wistar rats were randomly divided into four groups,one group as the normal control group,the remaining three groups rats were injected streptozotocin（STZ） into vena caudalis as the diabetic model.All the four group rats were punched in back to establish the diabetic ulcer model.The three group rats with diabetic ulcers were randomly divided into diabetic control group,vaseline group and SM group according to the blood glucose and body weight.The rats in SM group were treated with SM gauze compress,those in vaseline group treated with vaseline gauze compress,and those in normal control group and diabetic control group treated with physiological saline,then the content of serum IL-2 level and wound tumor necrosis factor-α（TNF-α） in the wound tissue were observed.Result： The diabetic ulcer wounds in the back of the rats in SM group healed faster than those in the rats in diabetic control group and vaseline group,and the difference was significant on the day 7,14,21 and 30 compared with diabetic control group,respectively;the contents of serum interleukin-2（IL-2） in the rats in SM group were lower than those in the rats in diabetic control group on the day 21 and 30,and the difference was significant;it was also lower than those in the rats in vaseline group on the day 21th and 30 and the difference was significant.The expression levels of TNF-α mRNA of the diabetic ulcer wounds in the rats in SM group were lower compared to those in the rats in diabetic control group on the day 21 and 30,and the difference was significant;it was also lower than those in the rats in vaseline group on the day,21 and 30 and the difference was significant.Conclusion： The Shengji Xiangpi mastic can promote the ulcer healing in diabetic rats,and it is closely related to regulating the immune-inflammatory response.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第18期279-282,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81072817)
