摘要
目的:从细胞增殖、周期和凋亡研究旋覆归连方治疗食管癌作用机制。方法:体外培养食管癌细胞株Eca9706,MTT法检测旋覆归连方抑制细胞增殖作用,观察其时效和量效;1×105/孔细胞接种于6孔板,加入15,25,60 mg·L-1的药物作用48 h,流式细胞仪检测细胞周期和凋亡。结果:旋覆归连方具有很强的抑制Eca9706增殖作用,具有剂量依赖性,IC5058.11mg·L-1;质量浓度15,25,60 mg·L-1在96 h内抑制率依时增加;早、晚期凋亡率用药组与阴性对照组相比明显增高(P<0.05);细胞G1/G0期比率也明显增加(P<0.05),60 mg·L-1组出现了明显的凋亡峰。结论:旋覆归连方能抑制食管癌细胞的增殖,具有浓度和时间依赖性,可能与其阻滞了细胞G1/G0期和诱导细胞凋亡有关。
Objective： From the cell proliferation,cycle and apoptosis researching the mechanism which Xuanfu Guilian prescription treats esophageal cancer.Method： Esophageal carcinoma cells（Eac9706） in vitro were cultured,and the cell proliferation inhibitory rate of Xuanfu Guilian prescription were detected with MTT assay to observe the time-and dose-effect;1 × 105cells / wells were seeded in 6 wells plate and respectively mixed in Xuanfu Guilian prescription extracts（15,25,60 mg·L-1）;after cultured 48 h,the cell cycle and apoptosis of Eac9706 were detected by flow cytometry（FCM）.Result： Xuanfu Guilian prescription can inhibit strongly the proliferation of Eca9706 cell,and have dose dependent,50% inhibition concentration（IC 50） 58.11 mg·L-1.The inhibition rate of concentration 15,25,60 mg·L-1increased on time in 96 h.Early and late apoptosis rate of treatment group were significantly increaser than the negative（P 0.05）.The cells rate of G 0 / G 1 also increased significantly（P 0.05）,and the 60 mg·L-1group appeared apoptosis peak.Conclusion： Xuanfu Guilian prescription can inhibit the proliferation of Eac9706 cells and there is in dose and time dependent,which mechanism may be related to drug block of cells to G 0 / G 1 phase and induce cell apoptosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第18期177-180,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173177)
关键词
旋覆归连方
食管癌
增殖
周期
凋亡
Xuanfu Guilian prescription
esophageal carcinoma cell Eac9706 cells
proliferation
cell cycle
apoptosis
