摘要
目的:探讨中药柴胡对人食管癌细胞株Eca-109的抑制作用。方法:体外培养人食管癌细胞株(Eca-109),分别给以不同浓度的柴胡溶液对体外培养的Eca-109细胞株进行干预。以MTT比色法测定柴胡溶液对Eca-109细胞株的抑制增殖的作用,计算其细胞增殖存活率。结果:MTT实验显示柴胡溶液可以明显抑制Eca-109细胞株的增殖。用药后Eca-109细胞增殖存活率下降幅度与剂量呈正相。结论:中药柴胡在体外具有抗肿瘤作用,其机制是抑制肿瘤细胞增殖,及抑制细胞的分化而达到抗肿瘤的目的。
Objective：To investigate the inhibitory effect of Radix Bupleuri on human esophageal cancer cell Eca-109.Methods：In vitro culture of human esophageal cancer cell （Eca-109）,were treated with different concentration of Radix Bupleurum solution on Eca-109 in vitro intervention.Inhibition of proliferation was measured Radix with MTT colorimetric method,to calculate the cell proliferation,survival rate.Results：MTT showed Bupleurum solution can significantly inhibit the proliferation of Eca-109 cells.After the proliferation of Eca-109 cell survival rate decreased with the dose of positive.Conclusion：Radix Bupleuri has antitum or effect in vitro,the mechanism is to inhibit the proliferation of tumor cells,and inhibited cell differentiati on and achieve the purpose of anti tumor.
出处
《实用中西医结合临床》
2013年第4期82-83,共2页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
福建厦门市科技局科研项目(项目编号:3502Z20077064)
关键词
柴胡
食管癌细胞
抑制作用
Radix bupleuri
Esophagus carcinoma cell
Inhibition
