摘要
采用西藏著名野生花卉蓝玉簪龙胆茎段为组织培养材料、通过多次试验后确定，蓝玉簪龙胆的启动培养中适宜的培养基为ＭＳ＋ＮＡＡ１．０ｍｇ／Ｌ、＋ＢＡ０．４～０．５ｍｇ／Ｌ、或者为ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ；继代培养阶段的适宜培养基为ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ；生根阶段的适宜培养基为１／２ＭＳ＋ＢＡ０．１ｍｇ／Ｌ＋ＩＢＡ０．５ ｍｇ／Ｌ。为蓝玉簪龙胆在组培条件下大量繁殖幼苗提供了条件，使蓝玉簪龙胆资源得到了有效保护和科学利用。
Using stem part of famous wild flowers and plains Gentiana veitchiorum in Tibet as material for tissue culture. after many experiments. the results show that MS+NAA1. 0 mg/L+BAO. 4 ~ 0. 5 mg/L or MS+BA 0. 5 mg/L+NAAO, 5 mg/L is the most effective in the first culture. MS+BAO. 5 mg/L+NAAO. 5 mg/L is the most fixable in the second culture, and l/2MS+BAO. 1 mg/L+ IBAO. 5 mg/L is the best in the rooting culture. On the other hand. it provides with effective techniques in protecting and using Gentiana veitchiorum.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2000年第6期93-94,共2页
Journal of Northeast Forestry University
关键词
蓝玉簪龙胆
茎段
组织培养
Gentiana veitchiorum
Stem part
Tissue culture
