摘要
以波尔山羊为胚胎供体、当地淮山羊为受体 ,以孕酮阴道栓作同期发情处理。采用促卵泡激素和促黄体激素对供体母羊作超数排卵处理。供体母羊与波尔种公羊首次交配后的 3.5 d和 5 .5 d分别于输卵管和子宫角回收胚胎。从 3头供体母羊共获 6 5枚胚胎 ,经鉴定分级 ,其中 5 3枚为可移植胚胎 ,分别移植至相应同期发情的 2 7头受体母羊的输卵管或子宫内 ,每头受体移入 1～ 2枚胚胎。经过 3个情期以上 ,其中 2 0头受体母羊未出现返情 ,受体妊娠率 74%。 7头母羊产双羔 ,9头母羊产单羔 ,共获 2 3头羔羊。妊娠受体产羔率为 80 % ,移植胚胎的发育率为43.4%。平均每头供体母羊获得纯种波尔山羊羔 7.7头。上述结果表明 ,此项技术在国际上已属较高水平 。
Boer does were used as embryo donors for transfer into local goats (Huai does).Donors and recipients were estrus synchronized with progesterone devices inserted for 17 days.The Boer does were superovulated with FSH administered as twice daily injection in decreasing doses for 3 or 4 days.The embryos were collected surgically on days 3 5 and 5 5 after first mating by mid ventral laparotomy.The synchronized recipients received 1～2 embryos that were transferred surgically by laparotomy as singles or in pairs into the oviduct(s) or the uterine horn(s) ipsilateral to the ovary with prominent corpus luteum development.A total of 65 embryos were recovered from three Boer does of which 53 were transferable embryos.All of them were tansferred to 27 recipients and pregnancy rate was 74%.Seven recipients gave birth to twin and nine recipients gave birth to single.7 7 kids each donor were obtained from one superovulation and embryo transfer treatment.It is concluded that commercial embryo transfer in Boer does is a viable technique for rapidly expanding superior donors.
出处
《江苏农业学报》
CSCD
北大核心
2000年第4期230-232,共3页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技厅资助项目
关键词
波尔山羊
超数排卵
同期发情
胚胎移植
Boer goat
superovulation
estrus synchronization
embryo transfe(
