摘要
以“郑红 5”等 6个甘薯品种为材料 ,通过离体培养诱导体细胞胚胎发生与植株再生。利用 RAPD分子标记方法 ,在 DNA水平上分析甘薯品种间遗传差异以及体细胞胚胎无性系的遗传变异。筛选了 45个随机引物 ,其中 7个引物 PCR扩增后在品种间和体细胞胚胎无性系中表现 RAPD多态性 ,并在相应胚性愈伤组织中检测到少数 RAPD变异位点。证明利用 RAPD分子标记方法鉴定甘薯体细胞胚胎发生遗传变异的可行性 ,以及为通过离体培养条件调控 (增加或减少 )
Six sweetpotato cultivars including “Zhenghong 5”were used for induction of somatic embryogenesis and plantlet regeneration in vitro.Genetic differences among cultivars and somaclonal variation in embryo derived plantlets had been analysed on DNA level using RAPD molecular marker.Out of 45 random primers screened using PCR amplification,7 primers revealed RAPD polymorphism for different cultivars and individual somaclones,and also presented a few variable RAPD loci in corresponding embryogenic callus.This study demonstrates the feasibility of using RAPD markers to identify genetic variation in sweetpotato somatic embryogenesis and provides evidence for regulating culture conditions in vitro to increase or decrease the genetic variation occurred in sweetpotato somatic embryogenesis.
出处
《江苏农业学报》
CSCD
北大核心
2000年第4期212-216,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省自然科学基金资助项目! (BK97193)
